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5a9x

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Structure of GDP bound BipA

Structural highlights

5a9x is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.8Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BIPA_ECO27 A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA (By similarity). GTPase that impacts interactions between enteropathogenic E.coli (EPEC) and epithelial cells and also has an effect on motility (PubMed:9622352).[HAMAP-Rule:MF_00849]^[1]

Publication Abstract from PubMed

BPI-inducible protein A (BipA) is a member of the family of ribosome-dependent translational GTPase (trGTPase) factors along with elongation factors G and 4 (EF-G and EF4). Despite being highly conserved in bacteria and playing a critical role in coordinating cellular responses to environmental changes, its structures (isolated and ribosome bound) remain elusive. Here, we present the crystal structures of apo form and GTP analog, GDP, and guanosine-3',5'-bisdiphosphate (ppGpp)-bound BipA. In addition to having a distinctive domain arrangement, the C-terminal domain of BipA has a unique fold. Furthermore, we report the cryo-electron microscopy structure of BipA bound to the ribosome in its active GTP form and elucidate the unique structural attributes of BipA interactions with the ribosome and A-site tRNA in the light of its possible function in regulating translation.

Structure of BipA in GTP form bound to the ratcheted ribosome.,Kumar V, Chen Y, Ero R, Ahmed T, Tan J, Li Z, Wong AS, Bhushan S, Gao YG Proc Natl Acad Sci U S A. 2015 Aug 17. pii: 201513216. PMID:26283392^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Farris M, Grant A, Richardson TB, O'Connor CD. BipA: a tyrosine-phosphorylated GTPase that mediates interactions between enteropathogenic Escherichia coli (EPEC) and epithelial cells. Mol Microbiol. 1998 Apr;28(2):265-79. PMID:9622352 doi:10.1046/j.1365-2958.1998.00793.x
↑ Kumar V, Chen Y, Ero R, Ahmed T, Tan J, Li Z, Wong AS, Bhushan S, Gao YG. Structure of BipA in GTP form bound to the ratcheted ribosome. Proc Natl Acad Sci U S A. 2015 Aug 17. pii: 201513216. PMID:26283392 doi:http://dx.doi.org/10.1073/pnas.1513216112

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5a9x"

@@ Line 1: / Line 1: @@
 ==Structure of GDP bound BipA==
-<StructureSection load='5a9x' size='340' side='right' caption='[[5a9x]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
+<StructureSection load='5a9x' size='340' side='right'caption='[[5a9x]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5a9x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A9X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5a9x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A9X FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a9v|5a9v]], [[5a9w|5a9w]], [[5a9y|5a9y]], [[5a9z|5a9z]], [[5aa0|5aa0]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a9x OCA], [http://pdbe.org/5a9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a9x RCSB], [http://www.ebi.ac.uk/pdbsum/5a9x PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a9x OCA], [https://pdbe.org/5a9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a9x RCSB], [https://www.ebi.ac.uk/pdbsum/5a9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a9x ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/BIPA_ECO27 BIPA_ECO27] A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA (By similarity). GTPase that impacts interactions between enteropathogenic E.coli (EPEC) and epithelial cells and also has an effect on motility (PubMed:9622352).[HAMAP-Rule:MF_00849]<ref>PMID:9622352</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 19: @@
 </div>
 <div class="pdbe-citations 5a9x" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Chen, Y]]
+[[Category: Escherichia coli]]
-[[Category: Ero, R]]
+[[Category: Large Structures]]
-[[Category: Gao, Y G]]
+[[Category: Chen Y]]
-[[Category: Kumar, V]]
+[[Category: Ero R]]
-[[Category: Li, Z]]
+[[Category: Gao Y-G]]
-[[Category: Bipa]]
+[[Category: Kumar V]]
-[[Category: Ribosomal protein]]
+[[Category: Li Z]]
-[[Category: Ribosome]]
-[[Category: Translational gtpase factor]]

5a9x

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Current revision

Structure of GDP bound BipA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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