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4yhu

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Yeast Prp3 C-terminal fragment 296-469

Structural highlights

4yhu is a 3 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRP3_YEAST Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.^[1]

Publication Abstract from PubMed

Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular mechanisms in pre-mRNA splicing are presently poorly understood. We show by structural and biochemical analyses that Prp3 contains a bipartite U4/U6 di-snRNA-binding region comprising an expanded ferredoxin-like fold, which recognizes a 3'-overhang of U6 snRNA, and a preceding peptide, which binds U4/U6 stem II. Phylogenetic analyses revealed that the single-stranded RNA-binding domain is exclusively found in Prp3 orthologs, thus qualifying as a spliceosome-specific RNA interaction module. The composite double-stranded/single-stranded RNA-binding region assembles cooperatively with Snu13 and Prp31 on U4/U6 di-snRNAs and inhibits Brr2-mediated U4/U6 di-snRNA unwinding in vitro. RNP-disrupting mutations in Prp3 lead to U4/U6*U5 tri-snRNP assembly and splicing defects in vivo. Our results reveal how Prp3 acts as an important bridge between U4/U6 and U5 in the tri-snRNP and comparison with a Prp24-U6 snRNA recycling complex suggests how Prp3 may be involved in U4/U6 re-assembly after splicing.

A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing.,Liu S, Mozaffari-Jovin S, Wollenhaupt J, Santos KF, Theuser M, Dunin-Horkawicz S, Fabrizio P, Bujnicki JM, Luhrmann R, Wahl MC Elife. 2015 Jul 10;4. doi: 10.7554/eLife.07320. PMID:26161500^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Anthony JG, Weidenhammer EM, Woolford JL Jr. The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP. RNA. 1997 Oct;3(10):1143-52. PMID:9326489
↑ Liu S, Mozaffari-Jovin S, Wollenhaupt J, Santos KF, Theuser M, Dunin-Horkawicz S, Fabrizio P, Bujnicki JM, Luhrmann R, Wahl MC. A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing. Elife. 2015 Jul 10;4. doi: 10.7554/eLife.07320. PMID:26161500 doi:http://dx.doi.org/10.7554/eLife.07320

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4yhu"

Categories: Large Structures | Saccharomyces cerevisiae | Liu S | Wahl MC

@@ Line 1: / Line 1: @@
 ==Yeast Prp3 C-terminal fragment 296-469==
-<StructureSection load='4yhu' size='340' side='right' caption='[[4yhu]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='4yhu' size='340' side='right'caption='[[4yhu]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4yhu]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YHU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4yhu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YHU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yhv|4yhv]], [[4yhw|4yhw]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yhu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yhu OCA], [http://pdbe.org/4yhu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yhu RCSB], [http://www.ebi.ac.uk/pdbsum/4yhu PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yhu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yhu OCA], [https://pdbe.org/4yhu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yhu RCSB], [https://www.ebi.ac.uk/pdbsum/4yhu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yhu ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PRP3_YEAST PRP3_YEAST]] Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.<ref>PMID:9326489</ref>
+[https://www.uniprot.org/uniprot/PRP3_YEAST PRP3_YEAST] Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.<ref>PMID:9326489</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 4yhu" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Liu, S]]
+[[Category: Large Structures]]
-[[Category: Wahl, M C]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Duf1115]]
+[[Category: Liu S]]
-[[Category: Ferredoxin-like fold]]
+[[Category: Wahl MC]]
-[[Category: Rna binding protein]]
-[[Category: Spliceosomal protein]]

4yhu

From Proteopedia

Current revision

Yeast Prp3 C-terminal fragment 296-469

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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