5any

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(New page: '''Unreleased structure''' The entry 5any is ON HOLD until sometime in the future Authors: Fox, J.M., Long, F., Edeling, M.A., Lin, H., Duijl-Richter, M., Fong, R.H., Kahle, K.M., Smit,...)
Current revision (06:19, 31 May 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5any is ON HOLD until sometime in the future
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==Electron cryo-microscopy of chikungunya virus in complex with neutralizing antibody Fab CHK265==
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<SX load='5any' size='340' side='right' viewer='molstar' caption='[[5any]], [[Resolution|resolution]] 16.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5any]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Chikungunya_virus Chikungunya virus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ANY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ANY FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5any FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5any OCA], [https://pdbe.org/5any PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5any RCSB], [https://www.ebi.ac.uk/pdbsum/5any PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5any ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q1H8W5_CHIKV Q1H8W5_CHIKV]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We screened a panel of mouse and human monoclonal antibodies (MAbs) against chikungunya virus and identified several with inhibitory activity against multiple alphaviruses. Passive transfer of broadly neutralizing MAbs protected mice against infection by chikungunya, Mayaro, and O'nyong'nyong alphaviruses. Using alanine-scanning mutagenesis, loss-of-function recombinant proteins and viruses, and multiple functional assays, we determined that broadly neutralizing MAbs block multiple steps in the viral lifecycle, including entry and egress, and bind to a conserved epitope on the B domain of the E2 glycoprotein. A 16 A resolution cryo-electron microscopy structure of a Fab fragment bound to CHIKV E2 B domain provided an explanation for its neutralizing activity. Binding to the B domain was associated with repositioning of the A domain of E2 that enabled cross-linking of neighboring spikes. Our results suggest that B domain antigenic determinants could be targeted for vaccine or antibody therapeutic development against multiple alphaviruses of global concern.
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Authors: Fox, J.M., Long, F., Edeling, M.A., Lin, H., Duijl-Richter, M., Fong, R.H., Kahle, K.M., Smit, J.M., Jin, J., Simmons, G., Doranz, B.J., Crowe, J.E., Fremont, D.H., Rossmann, M.G., Diamond, M.S.
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Broadly Neutralizing Alphavirus Antibodies Bind an Epitope on E2 and Inhibit Entry and Egress.,Fox JM, Long F, Edeling MA, Lin H, van Duijl-Richter MK, Fong RH, Kahle KM, Smit JM, Jin J, Simmons G, Doranz BJ, Crowe JE Jr, Fremont DH, Rossmann MG, Diamond MS Cell. 2015 Nov 19;163(5):1095-107. doi: 10.1016/j.cell.2015.10.050. Epub 2015 Nov, 6. PMID:26553503<ref>PMID:26553503</ref>
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Description: Electron cryo-microscopy of chikungunya virus in complex with neutralizing antibody Fab CHK265
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Kahle, K.M]]
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<div class="pdbe-citations 5any" style="background-color:#fffaf0;"></div>
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[[Category: Lin, H]]
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[[Category: Simmons, G]]
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==See Also==
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[[Category: Diamond, M.S]]
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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[[Category: Crowe, J.E]]
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== References ==
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[[Category: Jin, J]]
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<references/>
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[[Category: Fong, R.H]]
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__TOC__
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[[Category: Fremont, D.H]]
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</SX>
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[[Category: Edeling, M.A]]
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[[Category: Chikungunya virus]]
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[[Category: Fox, J.M]]
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[[Category: Large Structures]]
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[[Category: Rossmann, M.G]]
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[[Category: Mus musculus]]
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[[Category: Doranz, B.J]]
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[[Category: Crowe JE]]
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[[Category: Smit, J.M]]
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[[Category: Diamond MS]]
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[[Category: Duijl-Richter, M]]
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[[Category: Doranz BJ]]
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[[Category: Long, F]]
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[[Category: Duijl-Richter M]]
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[[Category: Edeling MA]]
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[[Category: Fong RH]]
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[[Category: Fox JM]]
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[[Category: Fremont DH]]
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[[Category: Jin J]]
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[[Category: Kahle KM]]
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[[Category: Lin H]]
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[[Category: Long F]]
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[[Category: Rossmann MG]]
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[[Category: Simmons G]]
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[[Category: Smit JM]]

Current revision

Electron cryo-microscopy of chikungunya virus in complex with neutralizing antibody Fab CHK265

5any, resolution 16.90Å

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