5dm9
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5dm9 is ON HOLD until Paper Publication Authors: Kobayashi, J., Nango, E. Description: XFEL structure of hen egg-white lysozyme solved using a drop...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==XFEL structure of hen egg-white lysozyme solved using a droplet injector at SACLA== | |
| + | <StructureSection load='5dm9' size='340' side='right'caption='[[5dm9]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5dm9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DM9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dm9 OCA], [https://pdbe.org/5dm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dm9 RCSB], [https://www.ebi.ac.uk/pdbsum/5dm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dm9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 microm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 A from <0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption. | ||
| - | + | Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography.,Mafune F, Miyajima K, Tono K, Takeda Y, Kohno JY, Miyauchi N, Kobayashi J, Joti Y, Nango E, Iwata S, Yabashi M Acta Crystallogr D Struct Biol. 2016 Apr;72(Pt 4):520-3. doi:, 10.1107/S2059798316001480. Epub 2016 Mar 24. PMID:27050131<ref>PMID:27050131</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5dm9" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kobayashi | + | |
| + | ==See Also== | ||
| + | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gallus gallus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kobayashi J]] | ||
| + | [[Category: Nango E]] | ||
Current revision
XFEL structure of hen egg-white lysozyme solved using a droplet injector at SACLA
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