5amv

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==Structural insights into the loss of catalytic competence in pectate lyase at low pH==
==Structural insights into the loss of catalytic competence in pectate lyase at low pH==
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<StructureSection load='5amv' size='340' side='right' caption='[[5amv]], [[Resolution|resolution]] 1.57&Aring;' scene=''>
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<StructureSection load='5amv' size='340' side='right'caption='[[5amv]], [[Resolution|resolution]] 1.57&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5amv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AMV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AMV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5amv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AMV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.57&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5amv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5amv OCA], [http://pdbe.org/5amv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5amv RCSB], [http://www.ebi.ac.uk/pdbsum/5amv PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5amv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5amv OCA], [https://pdbe.org/5amv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5amv RCSB], [https://www.ebi.ac.uk/pdbsum/5amv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5amv ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/PLY_BACSU PLY_BACSU]] Produces unsaturated products from polygalacturonate.
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[https://www.uniprot.org/uniprot/PLY_BACSU PLY_BACSU] Produces unsaturated products from polygalacturonate.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pectate lyase, a family 1 polysaccharide lyase, catalyses cleavage of the alpha-1,4 linkage of the polysaccharide homogalacturonan via an anti beta-elimination reaction. In the Michaelis complex two calcium ions bind between the C6 carboxylate of the d-galacturonate residue and enzyme aspartates at the active centre (+1 subsite), they withdraw electrons acidifying the C5 proton facilitating its abstraction by the catalytic arginine. Here we show that activity is lost at low pH because protonation of aspartates results in the loss of the two catalytic calcium-ions causing a profound failure to correctly organise the Michaelis complex.
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Structural insights into the loss of catalytic competence in pectate lyase activity at low pH.,Ali S, Sondergaard CR, Teixeira S, Pickersgill RW FEBS Lett. 2015 Sep 28. pii: S0014-5793(15)00838-8. doi:, 10.1016/j.febslet.2015.09.014. PMID:26420545<ref>PMID:26420545</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5amv" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ali, S]]
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[[Category: Bacillus subtilis]]
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[[Category: Pickersgill, R]]
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[[Category: Large Structures]]
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[[Category: Sondergaard, C]]
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[[Category: Ali S]]
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[[Category: Teixeira, S C.M]]
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[[Category: Pickersgill R]]
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[[Category: Bacillus subtilli]]
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[[Category: Sondergaard C]]
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[[Category: Catalytic activity]]
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[[Category: Teixeira SCM]]
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[[Category: Lyase]]
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[[Category: Pectate lyase]]
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Current revision

Structural insights into the loss of catalytic competence in pectate lyase at low pH

PDB ID 5amv

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