5fj8
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5fj8 is ON HOLD Authors: Hoffmann, N.A., Jakobi, A.J., Moreno-Morcillo, M., Glatt, S., Kosinski, J., Hagen, W.J., Sachse, C., Muller, C.W. Descript...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of yeast RNA polymerase III elongation complex at 3. 9 A== | |
| + | <SX load='5fj8' size='340' side='right' viewer='molstar' caption='[[5fj8]], [[Resolution|resolution]] 3.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5fj8]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FJ8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj8 OCA], [https://pdbe.org/5fj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fj8 RCSB], [https://www.ebi.ac.uk/pdbsum/5fj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fj8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RPAC1_YEAST RPAC1_YEAST] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Transcription of genes encoding small structured RNAs such as transfer RNAs, spliceosomal U6 small nuclear RNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase. Here we present the cryo-electron microscopy structures of the Saccharomyces cerevisiae Pol III elongating complex at 3.9 A resolution and the apo Pol III enzyme in two different conformations at 4.6 and 4.7 A resolution, respectively, which allow the building of a 17-subunit atomic model of Pol III. The reconstructions reveal the precise orientation of the C82-C34-C31 heterotrimer in close proximity to the stalk. The C53-C37 heterodimer positions residues involved in transcription termination close to the non-template DNA strand. In the apo Pol III structures, the stalk adopts different orientations coupled with closed and open conformations of the clamp. Our results provide novel insights into Pol III-specific transcription and the adaptation of Pol III towards its small transcriptional targets. | ||
| - | + | Molecular structures of unbound and transcribing RNA polymerase III.,Hoffmann NA, Jakobi AJ, Moreno-Morcillo M, Glatt S, Kosinski J, Hagen WJ, Sachse C, Muller CW Nature. 2015 Nov 25. doi: 10.1038/nature16143. PMID:26605533<ref>PMID:26605533</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5fj8" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: Jakobi | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Moreno-Morcillo | + | </SX> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Glatt S]] | ||
| + | [[Category: Hagen WJ]] | ||
| + | [[Category: Hoffmann NA]] | ||
| + | [[Category: Jakobi AJ]] | ||
| + | [[Category: Kosinski J]] | ||
| + | [[Category: Moreno-Morcillo M]] | ||
| + | [[Category: Muller CW]] | ||
| + | [[Category: Sachse C]] | ||
Current revision
Cryo-EM structure of yeast RNA polymerase III elongation complex at 3. 9 A
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