2rc3

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[[Image:2rc3.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of CBS domain, NE2398==
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|PDB= 2rc3 |SIZE=350|CAPTION= <scene name='initialview01'>2rc3</scene>, resolution 1.60&Aring;
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<StructureSection load='2rc3' size='340' side='right'caption='[[2rc3]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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<table><tr><td colspan='2'>[[2rc3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea_ATCC_19718 Nitrosomonas europaea ATCC 19718]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RC3 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE= NE2398 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 Nitrosomonas europaea ATCC 19718])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04623 CBS_pair_10], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04642 CBS_pair_29]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rc3 OCA], [https://pdbe.org/2rc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rc3 RCSB], [https://www.ebi.ac.uk/pdbsum/2rc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rc3 ProSAT], [https://www.topsan.org/Proteins/MCSG/2rc3 TOPSAN]</span></td></tr>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rc3 OCA], [http://www.ebi.ac.uk/pdbsum/2rc3 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2rc3 RCSB]</span>
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</table>
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}}
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== Function ==
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[https://www.uniprot.org/uniprot/Q82SE2_NITEU Q82SE2_NITEU]
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'''Crystal structure of CBS domain, NE2398'''
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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==Overview==
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<jmolCheckbox>
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We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/2rc3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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==About this Structure==
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<text>to colour the structure by Evolutionary Conservation</text>
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2RC3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea_atcc_19718 Nitrosomonas europaea atcc 19718]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RC3 OCA].
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rc3 ConSurf].
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==Reference==
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<div style="clear:both"></div>
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In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17982461 17982461]
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__TOC__
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[[Category: Nitrosomonas europaea atcc 19718]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dong, A.]]
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[[Category: Nitrosomonas europaea ATCC 19718]]
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[[Category: Edwards, A M.]]
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[[Category: Dong A]]
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[[Category: Joachimiak, A.]]
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[[Category: Edwards AM]]
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[[Category: Korniyenko, Y.]]
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[[Category: Joachimiak A]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Korniyenko Y]]
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[[Category: Savchenko, A.]]
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[[Category: Savchenko A]]
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[[Category: Walker, J R.]]
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[[Category: Walker JR]]
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[[Category: Xu, X.]]
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[[Category: Xu X]]
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[[Category: Yakunin, A.]]
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[[Category: Yakunin A]]
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[[Category: Zheng, H.]]
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[[Category: Zheng H]]
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[[Category: br]]
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[[Category: cbs domain]]
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[[Category: in situ proteolysis]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomic]]
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[[Category: protein structure initiative]]
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[[Category: psi-2]]
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[[Category: structural genomic]]
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[[Category: unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:45:51 2008''
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Current revision

Crystal structure of CBS domain, NE2398

PDB ID 2rc3

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OCA

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