Anthrax protective antigen
From Proteopedia
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| - | <StructureSection load='1acc' size=' | + | <StructureSection load='1acc' size='350' side='right' caption='Anthrax protective antigen (PA83) complex with Ca+2 ions (PDB code [[1acc]])' scene='48/485625/Cv/8'> |
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== Function == | == Function == | ||
| - | '''Anthrax protective antigen''' (PA) is the cell-binding part of the ''Bacillus anthracis'' anthrax toxin. The anthrax toxin is composed of the PA, lethal factor and edema factor. | + | '''Anthrax protective antigen''' (PA) is the cell-binding part of the ''Bacillus anthracis'' anthrax toxin. The anthrax toxin is composed of the PA, lethal factor and edema factor. The PA translocates the [[Anthrax Lethal Factor|lethal]] (LF) and [[Anthrax edema factor|edema]] factors (EF) into the cell. The 83kD form of PA '''(PA83)''' binds to the anthrax toxin receptor. A 20kD fragment (PA20) is cleaved off the PA83 and the remaining '''PA63''' assembles into ring-shaped oligomer which becomes a membrane pre-channel. The PA pre-channel binds the lethal and edema factors allowing them to penetrate the membrane via pH gradient. |
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| + | For more details see | ||
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| + | *[[Molecular Playground/Protective Antigen]]<br /> | ||
| + | *[[Anthrax Toxin Protein]]<br /> | ||
| + | *[[Toxins]]. | ||
== Structural highlights == | == Structural highlights == | ||
PA contains 4 domains:<br /> | PA contains 4 domains:<br /> | ||
| - | * <scene name='48/485625/Cv/3'>Domain 1 | + | * <scene name='48/485625/Cv/3'>Domain 1</scene> is the N terminal (in yellow). It contains the furin protease cleavage site enabling the release of PA20.<br /> |
| - | * Domain 2 is the heptamerization domain of PA63.<br /> | + | * <scene name='48/485625/Cv/4'>Domain 2</scene> (in cyan) is the heptamerization domain of PA63.<br /> |
| - | * Domain 3 forms the heptameric pore with domain 2 and enabling LF and EF to penetrate the cell.<br /> | + | * <scene name='48/485625/Cv/5'>Domain 3</scene> (in salmon) forms the heptameric pore with domain 2 and enabling LF and EF to penetrate the cell.<br /> |
| - | * Domain 4 is important for receptor recognition and pore formation. <br /> | + | * <scene name='48/485625/Cv/6'>Domain 4</scene> (in magenta) is important for receptor recognition and pore formation. <br /> |
| - | + | <scene name='48/485625/Cv/11'>Click here to see Ca binding site</scene>. <ref>PMID:9039918</ref> | |
| - | </ | + | |
==3D structures of anthrax protective antigen== | ==3D structures of anthrax protective antigen== | ||
| + | [[Anthrax protective antigen 3D structures]] | ||
| - | + | </StructureSection> | |
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| - | *PA domain 4 | ||
| - | + | == References == | |
| - | + | <references/> | |
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[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Petosa C, Collier RJ, Klimpel KR, Leppla SH, Liddington RC. Crystal structure of the anthrax toxin protective antigen. Nature. 1997 Feb 27;385(6619):833-8. PMID:9039918 doi:10.1038/385833a0
