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5dqz

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==Crystal Structure of Cas-DNA-PAM complex==
==Crystal Structure of Cas-DNA-PAM complex==
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<StructureSection load='5dqz' size='340' side='right' caption='[[5dqz]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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<StructureSection load='5dqz' size='340' side='right'caption='[[5dqz]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5dqz]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DQZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5dqz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DQZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5dlj|5dlj]], [[5dqu|5dqu]], [[5dqt|5dqt]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dqz OCA], [http://pdbe.org/5dqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dqz RCSB], [http://www.ebi.ac.uk/pdbsum/5dqz PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dqz OCA], [https://pdbe.org/5dqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dqz RCSB], [https://www.ebi.ac.uk/pdbsum/5dqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dqz ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CAS2_ECOLI CAS2_ECOLI]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity).<ref>PMID:21255106</ref> [[http://www.uniprot.org/uniprot/CAS1_ECOLI CAS1_ECOLI]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas1 is thought to be involved in CRISPR adaptation, the first stage of CRISPR immunity. Might be involved in the addition/removal of CRISPR spacers. Endonucleolytically cleaves linear ssRNA, ssDNA and short (34 base) dsDNA as well as branched DNA substrates such as Holliday junctions, replication forks and 5'-flap DNA substrates. Genetic interactions suggest Cas1 interacts with components of the RecBC and RuvB DNA repair systems.<ref>PMID:21255106</ref>
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[https://www.uniprot.org/uniprot/CAS1_ECOLI CAS1_ECOLI] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas1 is thought to be involved in CRISPR adaptation, the first stage of CRISPR immunity. Might be involved in the addition/removal of CRISPR spacers. Endonucleolytically cleaves linear ssRNA, ssDNA and short (34 base) dsDNA as well as branched DNA substrates such as Holliday junctions, replication forks and 5'-flap DNA substrates. Genetic interactions suggest Cas1 interacts with components of the RecBC and RuvB DNA repair systems.<ref>PMID:21255106</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 5dqz" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 5dqz" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[CRISPR-Cas Part II|CRISPR-Cas Part II]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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*[[Phospholipase A1|Phospholipase A1]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Li, J]]
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[[Category: Escherichia coli]]
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[[Category: Sheng, G]]
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[[Category: Escherichia coli K-12]]
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[[Category: Wang, J]]
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[[Category: Large Structures]]
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[[Category: Wang, M]]
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[[Category: Li J]]
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[[Category: Wang, Y]]
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[[Category: Sheng G]]
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[[Category: Yin, M]]
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[[Category: Wang J]]
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[[Category: Zhao, H]]
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[[Category: Wang M]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Wang Y]]
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[[Category: Protein-dna complex]]
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[[Category: Yin M]]
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[[Category: Zhao H]]

Current revision

Crystal Structure of Cas-DNA-PAM complex

PDB ID 5dqz

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