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4y5s

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==Structure of FtmOx1 with a-Ketoglutarate as co-substrate==
==Structure of FtmOx1 with a-Ketoglutarate as co-substrate==
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<StructureSection load='4y5s' size='340' side='right' caption='[[4y5s]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
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<StructureSection load='4y5s' size='340' side='right'caption='[[4y5s]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4y5s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y5S FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4y5s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y5S FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.543&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4y5t|4y5t]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Verruculogen_synthase Verruculogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.11.38 1.14.11.38] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y5s OCA], [https://pdbe.org/4y5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y5s RCSB], [https://www.ebi.ac.uk/pdbsum/4y5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y5s ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y5s OCA], [http://pdbe.org/4y5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y5s RCSB], [http://www.ebi.ac.uk/pdbsum/4y5s PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/FTMF_ASPFU FTMF_ASPFU]] Catalyzes the conversion of fumitremorgin B to verruculogen.<ref>PMID:19763315</ref>
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[https://www.uniprot.org/uniprot/FTMF_ASPFU FTMF_ASPFU] Catalyzes the conversion of fumitremorgin B to verruculogen.<ref>PMID:19763315</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many peroxy-containing secondary metabolites have been isolated and shown to provide beneficial effects to human health. Yet, the mechanisms of most endoperoxide biosyntheses are not well understood. Although endoperoxides have been suggested as key reaction intermediates in several cases, the only well-characterized endoperoxide biosynthetic enzyme is prostaglandin H synthase, a haem-containing enzyme. Fumitremorgin B endoperoxidase (FtmOx1) from Aspergillus fumigatus is the first reported alpha-ketoglutarate-dependent mononuclear non-haem iron enzyme that can catalyse an endoperoxide formation reaction. To elucidate the mechanistic details for this unique chemical transformation, we report the X-ray crystal structures of FtmOx1 and the binary complexes it forms with either the co-substrate (alpha-ketoglutarate) or the substrate (fumitremorgin B). Uniquely, after alpha-ketoglutarate has bound to the mononuclear iron centre in a bidentate fashion, the remaining open site for oxygen binding and activation is shielded from the substrate or the solvent by a tyrosine residue (Y224). Upon replacing Y224 with alanine or phenylalanine, the FtmOx1 catalysis diverts from endoperoxide formation to the more commonly observed hydroxylation. Subsequent characterizations by a combination of stopped-flow optical absorption spectroscopy and freeze-quench electron paramagnetic resonance spectroscopy support the presence of transient radical species in FtmOx1 catalysis. Our results help to unravel the novel mechanism for this endoperoxide formation reaction.
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Endoperoxide formation by an alpha-ketoglutarate-dependent mononuclear non-haem iron enzyme.,Yan W, Song H, Song F, Guo Y, Wu CH, Sae Her A, Pu Y, Wang S, Naowarojna N, Weitz A, Hendrich MP, Costello CE, Zhang L, Liu P, Jessie Zhang Y Nature. 2015 Nov 26;527(7579):539-43. doi: 10.1038/nature15519. Epub 2015 Nov 2. PMID:26524521<ref>PMID:26524521</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4y5s" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Verruculogen synthase]]
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[[Category: Aspergillus fumigatus Af293]]
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[[Category: Yan, W]]
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[[Category: Large Structures]]
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[[Category: Zhang, Y]]
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[[Category: Yan W]]
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[[Category: A-ketoglutarate]]
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[[Category: Zhang Y]]
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[[Category: Complex]]
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[[Category: Ftmox1]]
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[[Category: Oxidoreductase]]
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Current revision

Structure of FtmOx1 with a-Ketoglutarate as co-substrate

PDB ID 4y5s

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