5d94

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==Crystal structure of LC3-LIR peptide complex==
==Crystal structure of LC3-LIR peptide complex==
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<StructureSection load='5d94' size='340' side='right' caption='[[5d94]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
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<StructureSection load='5d94' size='340' side='right'caption='[[5d94]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5d94]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D94 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5d94]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D94 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d94 OCA], [http://pdbe.org/5d94 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d94 RCSB], [http://www.ebi.ac.uk/pdbsum/5d94 PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d94 OCA], [https://pdbe.org/5d94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d94 RCSB], [https://www.ebi.ac.uk/pdbsum/5d94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d94 ProSAT]</span></td></tr>
</table>
</table>
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== Disease ==
 
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[[http://www.uniprot.org/uniprot/FYCO1_HUMAN FYCO1_HUMAN]] Nuclear cataract. The disease is caused by mutations affecting the gene represented in this entry. Pathogenic mutations in FYCO1 can affect intracellular transport of autophagocytic vesicles from the perinuclear area to the periphery, leading to an accumulation of large numbers of vesicles and hence loss of lens transparency (PubMed:21636066).<ref>PMID:21636066</ref>
 
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes). [[http://www.uniprot.org/uniprot/FYCO1_HUMAN FYCO1_HUMAN]] May mediate microtubule plus end-directed vesicle transport.<ref>PMID:20100911</ref>
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[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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FYCO1 (FYVE and coiled-coil protein 1) is a motor adaptor that binds to PI3P (phosphatidylinositol 3-phosphate), to Rab7 and to LC3 to mediate transport of late endosomes and autophagosomes along microtubules in the plus end direction. We have previously shown that FYCO1 binds to LC3B via a 19-amino acid sequence containing a putative core LIR motif. Here, we show that FYCO1 preferentially binds to LC3A and -B. By peptide array-based two dimensional mutational scans of the binding to LC3B, we found FYCO1 to contain a C-terminally extended LIR domain. We determined the crystal structure of a complex between a 13-amino acid LIR peptide from FYCO1 and LC3B at 1.53 A resolution. By combining the structural information with mutational analyses both the basis for the C-terminal extended LIR and the specificity for LC3A/B binding were revealed. FYCO1 contains a 9-amino acid long F-type LIR motif. In addition to the canonical aromatic residue at position 1 and the hydrophobic residue at position 3, an acidic residue and a hydrophobic residue at positions 8 and 9, respectively, are important for efficient binding to LC3B explaining the C-terminal extension. The specificity for binding to LC3A/B is due to the interaction between D1285 in FYCO1 and H57 in LC3B. To address the functional significance of the LIR motif of FYCO1 we generated FYCO1 knock out cells that subsequently were reconstituted with GFP-FYCO1 wild type (WT) and LIR mutant constructs. Our data show that FYCO1 requires a functional LIR motif to facilitate efficient maturation of autophagosomes under basal conditions whereas starvation-induced autophagy was unaffected.
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FYCO1 Contains a C-terminally Extended, LC3A/B-preferring LC3-Interacting Region (LIR) Motif Required for Efficient Maturation of Autophagosomes During Basal Autophagy.,Olsvik HL, Lamark T, Takagi K, Bowitz Larsen K, Evjen G, Overvatn A, Mizushima T, Johansen T J Biol Chem. 2015 Oct 14. pii: jbc.M115.686915. PMID:26468287<ref>PMID:26468287</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Microtubule-associated protein 3D structures|Microtubule-associated protein 3D structures]]
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<div class="pdbe-citations 5d94" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Johansen, T]]
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[[Category: Homo sapiens]]
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[[Category: Mizushima, T]]
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[[Category: Large Structures]]
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[[Category: Takagi, K]]
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[[Category: Johansen T]]
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[[Category: Autophagy]]
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[[Category: Mizushima T]]
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[[Category: Fyco1]]
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[[Category: Takagi K]]
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[[Category: Lc3]]
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[[Category: Lir]]
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[[Category: Protein binding-peptide complex]]
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Current revision

Crystal structure of LC3-LIR peptide complex

PDB ID 5d94

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