2n6r
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==NMR structure of spider toxin U4-hexatoxin-Hi1a== | |
| + | <StructureSection load='2n6r' size='340' side='right'caption='[[2n6r]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2n6r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_infensa Hadronyche infensa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N6R FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6r OCA], [https://pdbe.org/2n6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6r RCSB], [https://www.ebi.ac.uk/pdbsum/2n6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6r ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/T41A_HADIN T41A_HADIN] Moderate insecticidal toxin with probable ion channel impairing activity. In vivo, induces irreversible paralysis in sheep blowflies (L.cuprina), leading to death at moderate doses (9.7 nmol/g).<ref>PMID:32398368</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Spiders are one of the most successful venomous animals, with more than 48,000 described species. Most spider venoms are dominated by cysteine-rich peptides with a diverse range of pharmacological activities. Some spider venoms contain thousands of unique peptides, but little is known about the mechanisms used to generate such complex chemical arsenals. We used an integrated transcriptomic, proteomic, and structural biology approach to demonstrate that the lethal Australian funnel-web spider produces 33 superfamilies of venom peptides and proteins. Twenty-six of the 33 superfamilies are disulfide-rich peptides, and we show that 15 of these are knottins that contribute >90% of the venom proteome. NMR analyses revealed that most of these disulfide-rich peptides are structurally related and range in complexity from simple to highly elaborated knottin domains, as well as double-knot toxins, that likely evolved from a single ancestral toxin gene. | ||
| - | + | Structural venomics reveals evolution of a complex venom by duplication and diversification of an ancient peptide-encoding gene.,Pineda SS, Chin YK, Undheim EAB, Senff S, Mobli M, Dauly C, Escoubas P, Nicholson GM, Kaas Q, Guo S, Herzig V, Mattick JS, King GF Proc Natl Acad Sci U S A. 2020 May 26;117(21):11399-11408. doi: , 10.1073/pnas.1914536117. Epub 2020 May 12. PMID:32398368<ref>PMID:32398368</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 2n6r" style="background-color:#fffaf0;"></div> |
| - | [[Category: Chin | + | == References == |
| - | [[Category: King | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Hadronyche infensa]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chin YK-Y]] | ||
| + | [[Category: King GF]] | ||
| + | [[Category: Pineda SS]] | ||
Current revision
NMR structure of spider toxin U4-hexatoxin-Hi1a
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