4zdt
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the RING finger domain of Slx1 in complex with the C-terminal domain of Slx4== | |
| + | <StructureSection load='4zdt' size='340' side='right'caption='[[4zdt]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4zdt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZDT FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zdt OCA], [https://pdbe.org/4zdt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zdt RCSB], [https://www.ebi.ac.uk/pdbsum/4zdt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zdt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SLX1_SCHPO SLX1_SCHPO] Catalytic subunit of the slx1-slx4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for stem-loop (SL) and splayed arm Y structures. Introduces a single-strand cut in duplex DNA on the 3' side of a double-strand/single-strand junction with respect to the single-strand moving 3' to 5' away from the junction. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. The complex initiates homologous recombination (HR) events, used to maintain rDNA copy number, in the rDNA repeats that are processed by a mechanism that requires rad22, but not rhp51. It is also required for suppression of methyl methanesulfonate (MMS) and UV-C irradiation hypersensitivity of the structural maintenance of chromosome (SMC) protein mutant, smc6-74, by overexpression of brc1. Has Holliday junction resolvase activity in vitro.[HAMAP-Rule:MF_03100]<ref>PMID:14528010</ref> <ref>PMID:15972456</ref> <ref>PMID:16467377</ref> <ref>PMID:17277362</ref> <ref>PMID:19596236</ref> | ||
| - | + | ==See Also== | |
| - | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: Qian | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| + | [[Category: Lian FM]] | ||
| + | [[Category: Qian CM]] | ||
| + | [[Category: Xie S]] | ||
Current revision
Crystal structure of the RING finger domain of Slx1 in complex with the C-terminal domain of Slx4
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