5a1s
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the sodium-dependent citrate symporter SeCitS form Salmonella enterica.== | |
| + | <StructureSection load='5a1s' size='340' side='right'caption='[[5a1s]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5a1s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A1S FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PTY:PHOSPHATIDYLETHANOLAMINE'>PTY</scene>, <scene name='pdbligand=UND:UNDECANE'>UND</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a1s OCA], [https://pdbe.org/5a1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a1s RCSB], [https://www.ebi.ac.uk/pdbsum/5a1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a1s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The common human pathogen Salmonella enterica takes up citrate as a nutrient via the sodium symporter SeCitS. Uniquely, our 2.5 A x-ray structure of the SeCitS dimer shows three different conformations of the active protomer. One protomer is in the outside-facing state. Two are in different inside-facing states. All three states resolve the substrates in their respective binding environments. Together with comprehensive functional studies on reconstituted proteoliposomes, the structures explain the transport mechanism in detail. Our results indicate a six-step process, with a rigid-body 31 degrees rotation of a helix bundle that translocates the bound substrates by 16 A across the membrane. Similar transport mechanisms may apply to a wide variety of related and unrelated secondary transporters, including important drug targets. | ||
| - | + | Mechanism of Na(+)-dependent citrate transport from the structure of an asymmetrical CitS dimer.,Wohlert D, Grotzinger MJ, Kuhlbrandt W, Yildiz O Elife. 2015 Dec 4;4. pii: e09375. doi: 10.7554/eLife.09375. PMID:26636752<ref>PMID:26636752</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5a1s" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kuhlbrandt | + | |
| - | [[Category: Woehlert | + | ==See Also== |
| - | [[Category: | + | *[[Symporter 3D structures|Symporter 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Salmonella enterica]] | ||
| + | [[Category: Groetzinger MJ]] | ||
| + | [[Category: Kuhlbrandt W]] | ||
| + | [[Category: Woehlert D]] | ||
| + | [[Category: Yildiz O]] | ||
Current revision
Crystal structure of the sodium-dependent citrate symporter SeCitS form Salmonella enterica.
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