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5azz
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5azz is ON HOLD until Paper Publication Authors: Watanabe, S., Okumura, M., Arai, K., Takei, T., Asahina, Y., Hojo, H., Iwaoka, M., Inaba, K. Descr...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of seleno-insulin== | |
| + | <StructureSection load='5azz' size='340' side='right'caption='[[5azz]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5azz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AZZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5azz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azz OCA], [https://pdbe.org/5azz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5azz RCSB], [https://www.ebi.ac.uk/pdbsum/5azz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5azz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Synthetic insulin analogues with a long lifetime are current drug targets for the therapy of diabetic patients. The replacement of the interchain disulfide with a diselenide bridge, which is more resistant to reduction and internal bond rotation, can enhance the lifetime of insulin in the presence of the insulin-degrading enzyme (IDE) without impairing the hormonal function. The [C7UA ,C7UB ] variant of bovine pancreatic insulin (BPIns) was successfully prepared by using two selenocysteine peptides (i.e., the C7U analogues of A- and B-chains, respectively). In a buffer solution at pH 10 they spontaneously assembled under thermodynamic control to the correct insulin fold. The selenoinsulin (Se-Ins) exhibited a bioactivity comparable to that of BPIns. Interestingly, degradation of Se-Ins with IDE was significantly decelerated (tau1/2 approximately 8 h vs. approximately 1 h for BPIns). The lifetime enhancement could be due to both the intrinsic stability of the diselenide bond and local conformational changes induced by the substitution. | ||
| - | + | Preparation of Selenoinsulin as a Long-Lasting Insulin Analogue.,Arai K, Takei T, Okumura M, Watanabe S, Amagai Y, Asahina Y, Moroder L, Hojo H, Inaba K, Iwaoka M Angew Chem Int Ed Engl. 2017 May 8;56(20):5522-5526. doi: 10.1002/anie.201701654., Epub 2017 Apr 10. PMID:28394477<ref>PMID:28394477</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5azz" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Insulin 3D Structures|Insulin 3D Structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: Okumura | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Watanabe | + | </StructureSection> |
| + | [[Category: Bos taurus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Arai K]] | ||
| + | [[Category: Asahina Y]] | ||
| + | [[Category: Hojo H]] | ||
| + | [[Category: Inaba K]] | ||
| + | [[Category: Iwaoka M]] | ||
| + | [[Category: Okumura M]] | ||
| + | [[Category: Takei T]] | ||
| + | [[Category: Watanabe S]] | ||
Current revision
Crystal structure of seleno-insulin
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Categories: Bos taurus | Large Structures | Arai K | Asahina Y | Hojo H | Inaba K | Iwaoka M | Okumura M | Takei T | Watanabe S
