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| - | [[Image:197d.gif|left|200px]] | |
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| - | {{Structure
| + | ==ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE== |
| - | |PDB= 197d |SIZE=350|CAPTION= <scene name='initialview01'>197d</scene>, resolution 2.190Å
| + | <StructureSection load='197d' size='340' side='right'caption='[[197d]], [[Resolution|resolution]] 2.19Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[197d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=197D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=197D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=197d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=197d OCA], [https://pdbe.org/197d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=197d RCSB], [https://www.ebi.ac.uk/pdbsum/197d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=197d ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=197d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=197d OCA], [http://www.ebi.ac.uk/pdbsum/197d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=197d RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Courseille C]] |
| - | '''ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE'''
| + | [[Category: Dautant A]] |
| - | | + | [[Category: Langlois D'Estaintot B]] |
| - | | + | [[Category: Precigoux G]] |
| - | ==Overview==
| + | |
| - | The X-ray crystal structure of the double-helical A-DNA octanucleotide d(GTACGTAC) has been solved by molecular replacement and refined to a resolution of 0.219 nm. The final R-factor is equal to 16.1% for 1516 observed reflections with F > 4 sigma(F). The sequence crystallizes as an A-DNA-type double helix in the orthorhombic space group P2(1)2(1)2, with one duplex molecule solvated by 66 water molecules in the asymmetric unit. Cell parameters are a = 3.860 nm, b = 5.082 nm, c = 2.174 nm. It is the first time that such a crystal form has been observed. This orthorhombic structure has been compared with the tetragonal structure of the same oligonucleotide. It adopts a bent structure with an unusual packing between symmetry-related molecules.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 197D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=197D OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Orthorhombic crystal structure of the A-DNA octamer d(GTACGTAC). Comparison with the tetragonal structure., Langlois d'Estaintot B, Dautant A, Courseille C, Precigoux G, Eur J Biochem. 1993 Apr 15;213(2):673-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8477741 8477741]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Courseille, C.]] | + | |
| - | [[Category: Dautant, A.]] | + | |
| - | [[Category: Estaintot, B Langlois D.]] | + | |
| - | [[Category: Precigoux, G.]] | + | |
| - | [[Category: a-dna]]
| + | |
| - | [[Category: double helix]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:30:09 2008''
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