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5ecy
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5ecy is ON HOLD Authors: Xu, K., Dedic, E., Brodersen, D.E. Description: Structure of the Shigella flexneri VapC mutant D98N crystal form 2 [[Categ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the Shigella flexneri VapC mutant D98N crystal form 2== | |
| + | <StructureSection load='5ecy' size='340' side='right'caption='[[5ecy]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ecy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ECY FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ecy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecy OCA], [https://pdbe.org/5ecy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ecy RCSB], [https://www.ebi.ac.uk/pdbsum/5ecy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ecy ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/VAPC_SHIFL VAPC_SHIFL] Toxic component of a toxin-antitoxin (TA) module. A tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Does not cleave tRNA(Met), tRNA(Arg2), tRNA(His), tRNA(Leu), tRNA(Phe) tRNA(Thr1), tRNA(Tyr) or tRNA(Val). Overexpression in E.coli inhibits translation, leads to loss of cell growth and degradation of tRNA(fMet); these effects are neutralized by expression of cognate antitoxin VapB. The VapB/VapC complex probably regulates transcription of its own promoter.<ref>PMID:19400780</ref> <ref>PMID:21502523</ref> Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity.<ref>PMID:19400780</ref> <ref>PMID:21502523</ref> | ||
| - | + | ==See Also== | |
| - | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: Dedic | + | [[Category: Large Structures]] |
| + | [[Category: Shigella flexneri]] | ||
| + | [[Category: Brodersen DE]] | ||
| + | [[Category: Dedic E]] | ||
| + | [[Category: Xu K]] | ||
Current revision
Structure of the Shigella flexneri VapC mutant D98N crystal form 2
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