This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1a63

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:21, 13 March 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1a63.gif|left|200px]]
 
-
{{Structure
+
==THE NMR STRUCTURE OF THE RNA BINDING DOMAIN OF E.COLI RHO FACTOR SUGGESTS POSSIBLE RNA-PROTEIN INTERACTIONS, 10 STRUCTURES==
-
|PDB= 1a63 |SIZE=350|CAPTION= <scene name='initialview01'>1a63</scene>
+
<StructureSection load='1a63' size='340' side='right'caption='[[1a63]]' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1a63]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A63 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a63 OCA], [https://pdbe.org/1a63 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a63 RCSB], [https://www.ebi.ac.uk/pdbsum/1a63 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a63 ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a63 OCA], [http://www.ebi.ac.uk/pdbsum/1a63 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a63 RCSB]</span>
+
[https://www.uniprot.org/uniprot/RHO_ECOLI RHO_ECOLI] Facilitates transcription termination by a mechanism that involves rho binding to the nascent RNA, activation of rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPAse which utilizes all four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01884]
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a63_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a63 ConSurf].
 +
<div style="clear:both"></div>
-
'''THE NMR STRUCTURE OF THE RNA BINDING DOMAIN OF E.COLI RHO FACTOR SUGGESTS POSSIBLE RNA-PROTEIN INTERACTIONS, 10 STRUCTURES'''
+
==See Also==
-
 
+
*[[Helicase 3D structures|Helicase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Rho protein is an essential hexameric RNA-DNA helicase that binds nascent mRNA transcripts and terminates transcription in a wide variety of eubacterial species. The NMR solution structure of the RNA binding domain of rho, rho130, is presented. This structure consists of two sub-domains, an N-terminal three-helix bundle and a C-terminal beta-barrel that is structurally similar to the oligosaccharide/oligonucleotide binding (OB) fold. Chemical shift changes of rho130 upon RNA binding and previous mutagenetic analyses of intact rho suggest that residues Asp 60, Phe 62, Phe 64, and Arg 66 are critical for binding and support the hypothesis that ssRNA/ssDNA binding is localized in the beta-barrel sub-domain. On the basis of these studies and the tertiary structure of rho130, we propose that residues Asp 60, Phe 62, Phe 64, Arg 66, Tyr 80, Lys 105, and Arg 109 participate in RNA-protein interactions.
+
[[Category: Large Structures]]
-
 
+
[[Category: Allison TJ]]
-
==About this Structure==
+
[[Category: Briercheck DM]]
-
1A63 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A63 OCA].
+
[[Category: Richardson JP]]
-
 
+
[[Category: Rule GS]]
-
==Reference==
+
[[Category: Wood TC]]
-
The NMR structure of the RNA binding domain of E. coli rho factor suggests possible RNA-protein interactions., Briercheck DM, Wood TC, Allison TJ, Richardson JP, Rule GS, Nat Struct Biol. 1998 May;5(5):393-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9587002 9587002]
+
-
[[Category: Escherichia coli]]
+
-
[[Category: Single protein]]
+
-
[[Category: Allison, T J.]]
+
-
[[Category: Briercheck, D M.]]
+
-
[[Category: Richardson, J P.]]
+
-
[[Category: Rule, G S.]]
+
-
[[Category: Wood, T C.]]
+
-
[[Category: ob fold]]
+
-
[[Category: rna binding domain]]
+
-
[[Category: termination]]
+
-
[[Category: transcription regulation]]
+
-
[[Category: transcription termination]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:34:04 2008''
+

Current revision

THE NMR STRUCTURE OF THE RNA BINDING DOMAIN OF E.COLI RHO FACTOR SUGGESTS POSSIBLE RNA-PROTEIN INTERACTIONS, 10 STRUCTURES

PDB ID 1a63

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1a63"
Personal tools