5fm1
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5fm1 is ON HOLD until sometime in the future Authors: Greenberg, C.H., Kollman, J., Zelter, A., Johnson, R., MacCoss, M.J., Davis, T.N., Agard, D.A....) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of gamma-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure== | |
| + | <SX load='5fm1' size='340' side='right' viewer='molstar' caption='[[5fm1]], [[Resolution|resolution]] 8.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5fm1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FM1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fm1 OCA], [https://pdbe.org/5fm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fm1 RCSB], [https://www.ebi.ac.uk/pdbsum/5fm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fm1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SPC97_YEAST SPC97_YEAST] Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Modeling protein complex structures based on distantly related homologues can be challenging due to poor sequence and structure conservation. Therefore, utilizing even low-resolution experimental data can significantly increase model precision and accuracy. Here, we present models of the two key functional states of the yeast gamma-tubulin small complex (gammaTuSC): one for the low-activity "open" state and another for the higher-activity "closed" state. Both models were computed based on remotely related template structures and cryo-EM density maps at 6.9A and 8.0A resolution, respectively. For each state, extensive sampling of alignments and conformations was guided by the fit to the corresponding cryo-EM density map. The resulting good-scoring models formed a tightly clustered ensemble of conformations in most regions. We found significant structural differences between the two states, primarily in the gamma-tubulin subunit regions where the microtubule binds. We also report a set of chemical cross-links that were found to be consistent with equilibrium between the open and closed states. The protocols developed here have been incorporated into our open-source Integrative Modeling Platform (IMP) software package (http://integrativemodeling.org), and can therefore be applied to many other systems. | ||
| - | + | Structure of gamma-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure.,Greenberg CH, Kollman J, Zelter A, Johnson R, MacCoss MJ, Davis TN, Agard DA, Sali A J Struct Biol. 2016 Jun;194(3):303-10. doi: 10.1016/j.jsb.2016.03.006. Epub 2016 , Mar 8. PMID:26968363<ref>PMID:26968363</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5fm1" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Tubulin 3D Structures|Tubulin 3D Structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </SX> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae]] | ||
| + | [[Category: Agard DA]] | ||
| + | [[Category: Davis TN]] | ||
| + | [[Category: Greenberg CH]] | ||
| + | [[Category: Johnson R]] | ||
| + | [[Category: Kollman J]] | ||
| + | [[Category: MacCoss MJ]] | ||
| + | [[Category: Sali A]] | ||
| + | [[Category: Zelter A]] | ||
Current revision
Structure of gamma-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure
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