1w6s

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[[Image:1w6s.gif|left|200px]]<br />
 
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<applet load="1w6s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1w6s, resolution 1.2&Aring;" />
 
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'''THE HIGH RESOLUTION STRUCTURE OF METHANOL DEHYDROGENASE FROM METHYLOBACTERIUM EXTORQUENS'''<br />
 
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==Overview==
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==The high resolution structure of methanol dehydrogenase from methylobacterium extorquens==
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The crystal structure of methanol dehydrogenase (MDH) from, Methylobacterium extorquens has been refined without stereochemical, restraints at a resolution of 1.2 A. The high-resolution data have defined, the conformation of the tricyclic pyrroloquinoline quinone (PQQ) cofactor, ring as entirely planar. The detailed definition of the active-site, geometry has shown many features that are similar to the, quinohaemo-protein alcohol dehydrogenases from Comamonas testosteroni and, Pseudomonas putida, both of which possess MDH-like and cytochrome c-like, domains. Conserved features between the two types of PQQ-containing enzyme, suggest a common pathway for electron transfer between MDH and its, physiological electron acceptor cytochrome cL. A pathway for proton, transfer from the active site to the bulk solvent is also suggested.
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<StructureSection load='1w6s' size='340' side='right'caption='[[1w6s]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1w6s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W6S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w6s OCA], [https://pdbe.org/1w6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w6s RCSB], [https://www.ebi.ac.uk/pdbsum/1w6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w6s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHM1_METEA DHM1_METEA] Catalyzes the oxidation of primary alcohols including methanol.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w6/1w6s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w6s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of methanol dehydrogenase (MDH) from Methylobacterium extorquens has been refined without stereochemical restraints at a resolution of 1.2 A. The high-resolution data have defined the conformation of the tricyclic pyrroloquinoline quinone (PQQ) cofactor ring as entirely planar. The detailed definition of the active-site geometry has shown many features that are similar to the quinohaemo-protein alcohol dehydrogenases from Comamonas testosteroni and Pseudomonas putida, both of which possess MDH-like and cytochrome c-like domains. Conserved features between the two types of PQQ-containing enzyme suggest a common pathway for electron transfer between MDH and its physiological electron acceptor cytochrome cL. A pathway for proton transfer from the active site to the bulk solvent is also suggested.
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==About this Structure==
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The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens.,Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):75-9. Epub 2004 Dec, 17. PMID:15608378<ref>PMID:15608378</ref>
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1W6S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens] with CA, PQQ and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W6S OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens., Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB, Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):75-9. Epub 2004 Dec, 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15608378 15608378]
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</div>
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[[Category: Alcohol dehydrogenase (acceptor)]]
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<div class="pdbe-citations 1w6s" style="background-color:#fffaf0;"></div>
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[[Category: Methylobacterium extorquens]]
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[[Category: Protein complex]]
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[[Category: Anthony, C.]]
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[[Category: Coates, L.]]
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[[Category: Cooper, J.B.]]
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[[Category: Erskine, P.T.]]
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[[Category: Gill, R.]]
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[[Category: Mohammed, F.]]
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[[Category: Williams, P.A.]]
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[[Category: Wood, S.P.]]
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[[Category: CA]]
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[[Category: GOL]]
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[[Category: PQQ]]
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[[Category: anisotropic]]
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[[Category: calcium-binding]]
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[[Category: electron transfer]]
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[[Category: methanol utilization]]
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[[Category: oxidoreductase]]
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[[Category: pqq]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:00:34 2007''
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==See Also==
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*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
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*[[Methanol dehydrogenase|Methanol dehydrogenase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Methylorubrum extorquens]]
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[[Category: Anthony C]]
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[[Category: Coates L]]
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[[Category: Cooper JB]]
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[[Category: Erskine PT]]
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[[Category: Gill R]]
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[[Category: Mohammed F]]
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[[Category: Williams PA]]
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[[Category: Wood SP]]

Current revision

The high resolution structure of methanol dehydrogenase from methylobacterium extorquens

PDB ID 1w6s

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