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| | ==The X-ray structure of the adduct formed in the reaction between bovine pancreatic ribonuclease and oxaliplatin== | | ==The X-ray structure of the adduct formed in the reaction between bovine pancreatic ribonuclease and oxaliplatin== |
| - | <StructureSection load='4s18' size='340' side='right' caption='[[4s18]], [[Resolution|resolution]] 2.27Å' scene=''> | + | <StructureSection load='4s18' size='340' side='right'caption='[[4s18]], [[Resolution|resolution]] 2.27Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4s18]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S18 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4s18]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S18 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PT:CYCLOHEXANE-1(R),2(R)-DIAMINE-PLATINUM(II)'>1PT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4s0q|4s0q]], [[4ot4|4ot4]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PT:CYCLOHEXANE-1(R),2(R)-DIAMINE-PLATINUM(II)'>1PT</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s18 OCA], [https://pdbe.org/4s18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s18 RCSB], [https://www.ebi.ac.uk/pdbsum/4s18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s18 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s18 OCA], [http://pdbe.org/4s18 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s18 RCSB], [http://www.ebi.ac.uk/pdbsum/4s18 PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN]] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref> | + | [https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4s18" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4s18" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pancreatic ribonuclease]] | + | [[Category: Bos taurus]] |
| - | [[Category: Merlino, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Bovine pancrea]] | + | [[Category: Merlino A]] |
| - | [[Category: Cleavage of rna]]
| + | |
| - | [[Category: Hydrolase]]
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| - | [[Category: Ribonuclease fold]]
| + | |
| - | [[Category: Rna]]
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| Structural highlights
Function
RNAS1_BOVIN Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.[1]
Publication Abstract from PubMed
Oxaliplatin and carboplatin are two platinum(II) drugs in widespread clinical use for the treatment of various types of cancers; yet, structural information on their interactions with proteins is scarce. Here, the X-ray structures of the adducts formed upon reaction of carboplatin and oxaliplatin with bovine pancreatic ribonuclease (RNase A) are reported and compared with results obtained for the structure of the RNase A-cisplatin adduct derived from isomorphous crystals, under the same experimental conditions. Additional details on the binding mode of these metallodrugs toward RNase A are provided by electrospray ionization mass spectrometry (ESI MS) measurements, thus offering insight on the occurring metal-protein interactions. Notably, while carboplatin and cisplatin mainly bind the side chain of Met29, oxaliplatin also binds the side chains of Asp14, of catalytically important His119 and, to a lesser extent, of His105. On the basis of the available data, a likely mechanism for oxaliplatin hydrolysis and binding to the protein is proposed. These results are potentially useful for a better understanding of the biological chemistry, toxicity and side effects of this important class of antitumor agents.
Interactions of carboplatin and oxaliplatin with proteins: Insights from X-ray structures and mass spectrometry studies of their ribonuclease A adducts.,Messori L, Marzo T, Merlino A J Inorg Biochem. 2015 Jul 21. pii: S0162-0134(15)30038-6. doi:, 10.1016/j.jinorgbio.2015.07.011. PMID:26239545[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT. Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 1995 Mar;8(3):261-73. PMID:7479688
- ↑ Messori L, Marzo T, Merlino A. Interactions of carboplatin and oxaliplatin with proteins: Insights from X-ray structures and mass spectrometry studies of their ribonuclease A adducts. J Inorg Biochem. 2015 Jul 21. pii: S0162-0134(15)30038-6. doi:, 10.1016/j.jinorgbio.2015.07.011. PMID:26239545 doi:http://dx.doi.org/10.1016/j.jinorgbio.2015.07.011
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