5ep5

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(New page: ==The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.== <StructureSection load='5ep5' size='340' side='right' caption='5ep5, [[Resol...)
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==The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.==
==The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.==
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<StructureSection load='5ep5' size='340' side='right' caption='[[5ep5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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<StructureSection load='5ep5' size='340' side='right'caption='[[5ep5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5ep5]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EP5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5ep5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EP5 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m20|4m20]], [[5eo5|5eo5]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ep5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ep5 OCA], [http://pdbe.org/5ep5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ep5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ep5 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ep5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ep5 OCA], [https://pdbe.org/5ep5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ep5 RCSB], [https://www.ebi.ac.uk/pdbsum/5ep5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ep5 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0H3JUJ1_STAAM A0A0H3JUJ1_STAAM]
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Staphylococcus aureus is the causative agent of many diseases, including meningitis, bacteraemia, pneumonia, food poisoning and toxic shock syndrome. Structural characterization of the PaaI-like thioesterase SAV0944 (SaPaaI) from S. aureus subsp. aureus Mu50 will aid in understanding its potential as a new therapeutic target by knowledge of its molecular details and cellular functions. Here, the recombinant expression, purification and crystallization of SaPaaI thioesterase from S. aureus are reported. This protein initially crystallized with the ligand coenzyme A using the hanging-drop vapour-diffusion technique with condition No. 40 of Crystal Screen from Hampton Research at 296 K. Optimal final conditions consisting of 24% PEG 4000, 100 mM sodium citrate pH 6.5, 12% 2-propanol gave single diffraction-quality crystals. These crystals diffracted to beyond 2 A resolution at the Australian Synchrotron and belonged to space group P12(1)1, with unit-cell parameters a = 44.05, b = 89.05, c = 60.74 A, beta = 100.5 degrees . Initial structure determination and refinement gave an R factor and R(free) of 17.3 and 22.0%, respectively, confirming a positive solution in obtaining phases using molecular replacement.
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Expression, purification, crystallization and preliminary X-ray analysis of the PaaI-like thioesterase SAV0944 from Staphylococcus aureus.,Khandokar YB, Roman N, Smith KM, Srivastava P, Forwood JK Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):244-7. doi:, 10.1107/S2053230X14000338. Epub 2014 Jan 22. PMID:24637766<ref>PMID:24637766</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5ep5" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Forwood, J K]]
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[[Category: Large Structures]]
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[[Category: Khandokar, Y B]]
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[[Category: Staphylococcus aureus subsp. aureus Mu50]]
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[[Category: Srivastava, P]]
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[[Category: Forwood JK]]
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[[Category: Coenzyme a]]
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[[Category: Khandokar YB]]
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[[Category: Hydrolase]]
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[[Category: Srivastava P]]
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[[Category: Hypothetical protein sav0944]]
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[[Category: Staphylococcus aureus]]
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[[Category: Thioesterase]]
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Current revision

The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.

PDB ID 5ep5

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