1ad2

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[[Image:1ad2.gif|left|200px]]
 
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{{Structure
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==RIBOSOMAL PROTEIN L1 MUTANT WITH SERINE 179 REPLACED BY CYSTEINE==
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|PDB= 1ad2 |SIZE=350|CAPTION= <scene name='initialview01'>1ad2</scene>, resolution 1.9&Aring;
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<StructureSection load='1ad2' size='340' side='right'caption='[[1ad2]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1ad2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AD2 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ad2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ad2 OCA], [https://pdbe.org/1ad2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ad2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ad2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ad2 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ad2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ad2 OCA], [http://www.ebi.ac.uk/pdbsum/1ad2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ad2 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/RL1_THETH RL1_THETH] The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release (By similarity). Binds directly to 23S rRNA.[HAMAP-Rule:MF_01318] Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA (By similarity).[HAMAP-Rule:MF_01318]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/1ad2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ad2 ConSurf].
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<div style="clear:both"></div>
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'''RIBOSOMAL PROTEIN L1 MUTANT WITH SERINE 179 REPLACED BY CYSTEINE'''
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==See Also==
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*[[Ribosomal protein L1|Ribosomal protein L1]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of the mutant S179C of the ribosomal protein L1 from Thermus thermophilus has been determined at 1.9 A resolution. The mutant molecule displays a small but significant opening of the cavity between the two domains. The domain movement seems to be facilitated by the flexibility of at least two conserved glycines. These glycines may be necessary for the larger conformational change needed for an induced fit mechanism upon binding RNA. The domain movement makes a disulfide bridge possible between the incorporated cysteines in two monomers of the mutant L1.
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[[Category: Large Structures]]
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==About this Structure==
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1AD2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AD2 OCA].
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==Reference==
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A mutant form of the ribosomal protein L1 reveals conformational flexibility., Unge J, Al-Karadaghi S, Liljas A, Jonsson BH, Eliseikina I, Ossina N, Nevskaya N, Fomenkova N, Garber M, Nikonov S, FEBS Lett. 1997 Jul 7;411(1):53-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9247141 9247141]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Al-Karadaghi, S.]]
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[[Category: Al-Karadaghi S]]
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[[Category: Eliseikina, I.]]
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[[Category: Eliseikina I]]
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[[Category: Fomenkova, N.]]
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[[Category: Fomenkova N]]
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[[Category: Garber, M.]]
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[[Category: Garber M]]
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[[Category: Jonsson, B H.]]
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[[Category: Jonsson B-H]]
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[[Category: Liljas, A.]]
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[[Category: Liljas A]]
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[[Category: Nevskaya, N.]]
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[[Category: Nevskaya N]]
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[[Category: Nikonov, S.]]
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[[Category: Nikonov S]]
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[[Category: Ossina, N.]]
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[[Category: Ossina N]]
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[[Category: Unge, J.]]
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[[Category: Unge J]]
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[[Category: mutant]]
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[[Category: protein synthesis]]
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[[Category: ribosomal protein]]
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[[Category: rna binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:37:46 2008''
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Current revision

RIBOSOMAL PROTEIN L1 MUTANT WITH SERINE 179 REPLACED BY CYSTEINE

PDB ID 1ad2

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