Arginine repressor

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== Function ==
== Function ==
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'''Arginine repressor''' (ArgR) is a prokaryotic repressor which regulates the arginine deiminase pathway. In this pathway, arginine is metabolized to form ammonia, CO2 and ATP. The ArgR releases the expression of the arginine deiminase pathway in the presence of arginine. The genes controlled by ArgR are not found in a single operon. While repressors are usually active as dimers, ArgR is a hexamer and binds to 2 palindromic DNA sites called ARG box in its N terminal domain.
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'''Arginine repressor''' (ArgR) is a prokaryotic repressor which regulates the arginine deiminase pathway. In this pathway, arginine is metabolized to form ammonia, CO2 and ATP. The ArgR releases the expression of the arginine deiminase pathway in the presence of arginine. The genes controlled by ArgR are not found in a single operon. While repressors are usually active as dimers, ArgR is a hexamer and binds to 2 palindromic DNA sites called ARG box in its N terminal domain.<ref>PMID:10331868</ref>
== Structural highlights ==
== Structural highlights ==
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The structure of ArgR shows a DNA-binding domain at the acidic N-terminal and a basic C-terminal domain which contains the <scene name='55/554907/Cv/5'>intersubunit interaction sites</scene> and the <scene name='55/554907/Cv/3'>Arg binding site</scene>.
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The structure of ArgR shows a DNA-binding domain at the acidic N-terminal and a basic C-terminal domain which contains the <scene name='55/554907/Cv/5'>intersubunit interaction sites</scene> and the <scene name='55/554907/Cv/10'>Arg binding site</scene>. Every Arg ligand interacts <scene name='55/554907/Cv/11'>with residues from 3 subunits</scene>.<ref>PMID:8594204</ref>
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</StructureSection>
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==3D structures of arginine repressor==
==3D structures of arginine repressor==
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[[Arginine repressor 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Arginine repressor
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**[[1b4a]] – GsArgR – ''Geobacillus stearothermophilus'' <br />
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**[[1f9n]] – BsArgR – ''Bacillus subtilis'' <br />
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**[[3v4g]] – ArgR – ''Vibrio vulnificus'' <br />
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**[[3ere]] – MtArgR + DNA – ''Mycobacterium tuberculosis'' <br />
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**[[3fhz]], [[3laj]] – MtArgR + DNA + Arg <br />
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**[[3lap]] – MtArgR + DNA + canavanine <br />
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*Arginine repressor N terminal
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**[[1aoy]] – EcArgR N terminal - ''Escherichia coli'' - NMR<br />
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**[[1b4b]] – BsArgR N terminal <br />
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**[[2p5l]] – BsArgR N terminal + DNA <br />
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*Arginine repressor C terminal
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**[[1xxc]] – EcArgR C terminal <br />
 
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**[[2p5k]] – GsArgR C terminal <br />
 
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**[[3bue]] – MtArgR C terminal <br />
 
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**[[1xxa]], [[1xxb]] – EcArgR C terminal + Arg <br />
 
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**[[2p5m]] – BsArgR C terminal (mutant) + Arg <br />
 
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**[[2zfz]], [[3cag]] – MtArgR C terminal + Arg <br />
 
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}}
 
== References ==
== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

E. coli structure of arginine repressor C terminal hexamer complex with arginine (PDB entry 1xxb)

Drag the structure with the mouse to rotate

References

  1. ↑ Ni J, Sakanyan V, Charlier D, Glansdorff N, Van Duyne GD. Structure of the arginine repressor from Bacillus stearothermophilus. Nat Struct Biol. 1999 May;6(5):427-32. PMID:10331868 doi:http://dx.doi.org/10.1038/8229
  2. ↑ Van Duyne GD, Ghosh G, Maas WK, Sigler PB. Structure of the oligomerization and L-arginine binding domain of the arginine repressor of Escherichia coli. J Mol Biol. 1996 Feb 23;256(2):377-91. PMID:8594204 doi:http://dx.doi.org/10.1006/jmbi.1996.0093

Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel

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