1ae7

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[[Image:1ae7.gif|left|200px]]
 
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{{Structure
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==NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2==
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|PDB= 1ae7 |SIZE=350|CAPTION= <scene name='initialview01'>1ae7</scene>, resolution 2.0&Aring;
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<StructureSection load='1ae7' size='340' side='right'caption='[[1ae7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE= <scene name='pdbsite=CAL:Site+*Cal*+Comprises+Those+Residues+Forming+The+Ca2++Bin+...'>CAL</scene> and <scene name='pdbsite=CNR:Site+*Cnr*+Comprises+Those+Residues+Forming+The+Catalyti+...'>CNR</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1ae7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Notechis_scutatus_scutatus Notechis scutatus scutatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE7 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae7 OCA], [https://pdbe.org/1ae7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae7 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae7 OCA], [http://www.ebi.ac.uk/pdbsum/1ae7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ae7 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PA2B_NOTSC PA2B_NOTSC] Snake venom phospholipase A2 (PLA2) that inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini. Is directly toxic to skeletal muscle upon local application in vivo (dystrophic effect). Also has direct nephrotoxicity in experimental mice; a single subcutaneous dose (1.38 ug/kg) produces renal tubular and glomerular damage within 24 hours (PubMed:7580101). PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structure of notexin has been solved by molecular replacement methods. The structure has been refined at 2.0 A resolution to a crystallographic R-value of 16.5% with good stereo-chemistry. The core of the protein is very similar to other phospholipase A2s (PLA2 s) but several parts of the molecule are distinctly different. The most significant differences from PLA2 s from bovine pancreas and rattlesnake occur in the stretches 56-80 and 85-89. Residue 69, which has been shown to be important for phospholipase binding, has a different conformation and different interactions than in other known PLA2s. The C alpha positions for residues 86-88 differ by about 6 A from both the bovine and the rattlesnake enzyme. The crystals contain no Ca2+ ions. Instead, a water molecule occupies the calcium site.
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'''NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2'''
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The three-dimensional structure of notexin, a presynaptic neurotoxic phospholipase A2 at 2.0 A resolution.,Westerlund B, Nordlund P, Uhlin U, Eaker D, Eklund H FEBS Lett. 1992 Apr 20;301(2):159-64. PMID:1568473<ref>PMID:1568473</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ae7" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The three-dimensional structure of notexin has been solved by molecular replacement methods. The structure has been refined at 2.0 A resolution to a crystallographic R-value of 16.5% with good stereo-chemistry. The core of the protein is very similar to other phospholipase A2s (PLA2 s) but several parts of the molecule are distinctly different. The most significant differences from PLA2 s from bovine pancreas and rattlesnake occur in the stretches 56-80 and 85-89. Residue 69, which has been shown to be important for phospholipase binding, has a different conformation and different interactions than in other known PLA2s. The C alpha positions for residues 86-88 differ by about 6 A from both the bovine and the rattlesnake enzyme. The crystals contain no Ca2+ ions. Instead, a water molecule occupies the calcium site.
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1AE7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Notechis_scutatus_scutatus Notechis scutatus scutatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE7 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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The three-dimensional structure of notexin, a presynaptic neurotoxic phospholipase A2 at 2.0 A resolution., Westerlund B, Nordlund P, Uhlin U, Eaker D, Eklund H, FEBS Lett. 1992 Apr 20;301(2):159-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1568473 1568473]
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[[Category: Notechis scutatus scutatus]]
[[Category: Notechis scutatus scutatus]]
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[[Category: Phospholipase A(2)]]
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[[Category: Eaker D]]
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[[Category: Single protein]]
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[[Category: Eklund H]]
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[[Category: Eaker, D.]]
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[[Category: Nordlund P]]
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[[Category: Eklund, H.]]
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[[Category: Uhlin U]]
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[[Category: Nordlund, P.]]
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[[Category: Westerlund B]]
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[[Category: Uhlin, U.]]
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[[Category: Westerlund, B.]]
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[[Category: hydrolase]]
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[[Category: lipid degradation]]
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[[Category: phospholipase a2]]
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[[Category: presynaptic neurotoxin]]
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[[Category: venom]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:38:20 2008''
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Current revision

NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2

PDB ID 1ae7

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