5ezk

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m (Protected "5ezk" [edit=sysop:move=sysop])
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'''Unreleased structure'''
 
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The entry 5ezk is ON HOLD
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==RNA polymerase model placed by Molecular replacement into X-ray diffraction map of DNA-bound RNA Polymerase-Sigma 54 holoenzyme complex.==
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<StructureSection load='5ezk' size='340' side='right'caption='[[5ezk]], [[Resolution|resolution]] 8.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5ezk]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EZK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 8.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ezk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezk OCA], [https://pdbe.org/5ezk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ezk RCSB], [https://www.ebi.ac.uk/pdbsum/5ezk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ezk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOA_ECOLI RPOA_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transcription by RNA polymerase (RNAP) in bacteria requires specific promoter recognition by sigma factors. The major variant sigma factor (sigma(54)) initially forms a transcriptionally silent complex requiring specialized adenosine triphosphate-dependent activators for initiation. Our crystal structure of the 450-kilodalton RNAP-sigma(54) holoenzyme at 3.8 angstroms reveals molecular details of sigma(54) and its interactions with RNAP. The structure explains how sigma(54) targets different regions in RNAP to exert its inhibitory function. Although sigma(54) and the major sigma factor, sigma(70), have similar functional domains and contact similar regions of RNAP, unanticipated differences are observed in their domain arrangement and interactions with RNAP, explaining their distinct properties. Furthermore, we observe evolutionarily conserved regulatory hotspots in RNAPs that can be targeted by a diverse range of mechanisms to fine tune transcription.
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Authors: Darbari, V.C., Yang, Y., Lu, D., Zhang, N., Glyde, R., Wang, Y., Murakami, K.S., Buck, M., Zhang, X.
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TRANSCRIPTION. Structures of the RNA polymerase-sigma54 reveal new and conserved regulatory strategies.,Yang Y, Darbari VC, Zhang N, Lu D, Glyde R, Wang YP, Winkelman JT, Gourse RL, Murakami KS, Buck M, Zhang X Science. 2015 Aug 21;349(6250):882-5. doi: 10.1126/science.aab1478. PMID:26293966<ref>PMID:26293966</ref>
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Description: RNA polymerase model placed by Molecular replacement into X-ray diffraction map of DNA-bound RNA Polymerase-Sigma 54 holoenzyme complex.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Yang, Y]]
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<div class="pdbe-citations 5ezk" style="background-color:#fffaf0;"></div>
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[[Category: Darbari, V.C]]
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== References ==
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[[Category: Wang, Y]]
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<references/>
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[[Category: Zhang, X]]
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__TOC__
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[[Category: Murakami, K.S]]
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</StructureSection>
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[[Category: Zhang, N]]
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[[Category: Escherichia coli]]
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[[Category: Glyde, R]]
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[[Category: Large Structures]]
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[[Category: Lu, D]]
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[[Category: Buck M]]
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[[Category: Buck, M]]
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[[Category: Darbari VC]]
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[[Category: Glyde R]]
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[[Category: Lu D]]
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[[Category: Murakami KS]]
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[[Category: Wang Y]]
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[[Category: Yang Y]]
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[[Category: Zhang N]]
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[[Category: Zhang X]]

Current revision

RNA polymerase model placed by Molecular replacement into X-ray diffraction map of DNA-bound RNA Polymerase-Sigma 54 holoenzyme complex.

PDB ID 5ezk

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