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1sfv

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[[Image:1sfv.gif|left|200px]]<br />
 
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<applet load="1sfv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sfv" />
 
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'''PORCINE PANCREAS PHOSPHOLIPASE A2, NMR, MINIMIZED AVERAGE STRUCTURE'''<br />
 
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==Overview==
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==PORCINE PANCREAS PHOSPHOLIPASE A2, NMR, MINIMIZED AVERAGE STRUCTURE==
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The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present, in a 40 kDa ternary complex with micelles and a competitive inhibitor, has, been determined using multidimensional heteronuclear NMR spectroscopy. The, structure of the protein (124 residues) is based on 1854 constraints, comprising 1792 distance and 62 phi torsion angle constraints. A total of, 18 structures was calculated using a combined approach of distance, geometry and restrained molecular dynamics. The atomic rms distribution, about the mean coordinate positions for residues 1-62 and 72-124 is 0.75, +/- 0.09 A for the backbone atoms and 1.14 +/- 0.10 A for all atoms. The, rms difference between the averaged minimized NMR structures of the free, PLA2 and PLA2 in the ternary complex is 3.5 A for the backbone atoms and, 4.0 A for all atoms. Large differences occur for the calcium-binding loop, and the surface loop from residues 62 through 72. The most important, difference is found for the first three residues of the N-terminal, alpha-helix. Whereas free in solution Ala1, Leu2 and Trp3 are disordered, with the alpha-helical conformation with the alpha-amino group buried, inside the protein. As a consequence, the important conserved hydrogen, bonding network which is also seen in the crystal structures is present, only in the ternary complex, but not in free PLA2. Thus, the NMR structure, of the N-terminal region (as well as the calcium-binding loop and the, surface loop) of PLA2 in the ternary complex resembles that of the crystal, structure. Comparison of the NMR structures of the free enzyme and the, enzyme in the ternary complex indicates that conformational changes play a, role in the interfacial activation of PLA2.
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<StructureSection load='1sfv' size='340' side='right'caption='[[1sfv]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sfv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfv OCA], [https://pdbe.org/1sfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sfv RCSB], [https://www.ebi.ac.uk/pdbsum/1sfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA21B_PIG PA21B_PIG] PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/1sfv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sfv ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SFV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Structure known Active Site: CA. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SFV OCA].
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Solution structure of porcine pancreatic phospholipase A2 complexed with micelles and a competitive inhibitor., van den Berg B, Tessari M, Boelens R, Dijkman R, Kaptein R, de Haas GH, Verheij HM, J Biomol NMR. 1995 Feb;5(2):110-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7703697 7703697]
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[[Category: Large Structures]]
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[[Category: Phospholipase A(2)]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Berg, B.Van.Den.]]
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[[Category: Boelens R]]
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[[Category: Boelens, R.]]
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[[Category: De Haas GH]]
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[[Category: Dijkman, R.]]
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[[Category: Dijkman R]]
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[[Category: Haas, G.H.De.]]
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[[Category: Kaptein R]]
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[[Category: Kaptein, R.]]
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[[Category: Tessari M]]
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[[Category: Tessari, M.]]
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[[Category: Van Den Berg B]]
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[[Category: Verheij, H.M.]]
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[[Category: Verheij HM]]
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[[Category: CA]]
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[[Category: phosphatide-2-acyl-hydrolase]]
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[[Category: phospholipase a2]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:01:20 2007''
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Current revision

PORCINE PANCREAS PHOSPHOLIPASE A2, NMR, MINIMIZED AVERAGE STRUCTURE

PDB ID 1sfv

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