5fou
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5fou is ON HOLD Authors: Scott, D.E., Marsh, M., Blundell, T.L., Abell, C., Hyvonen, M. Description: HUMANISED MONOMERIC RADA IN COMPLEX WITH FHPA ...) |
|||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==HUMANISED MONOMERIC RADA IN COMPLEX WITH FHPA TETRAPEPTIDE== | |
| + | <StructureSection load='5fou' size='340' side='right'caption='[[5fou]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5fou]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FOU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fou OCA], [https://pdbe.org/5fou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fou RCSB], [https://www.ebi.ac.uk/pdbsum/5fou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fou ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RADA_PYRFU RADA_PYRFU] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RAD51 is a recombinase involved in the homologous recombination of double-strand breaks in DNA. RAD51 forms oligomers by binding to another molecule of RAD51 via an 'FxxA' motif, and the same recognition sequence is similarly utilised to bind BRCA2. We have tabulated the effects of mutation of this sequence, across a variety of experimental methods and from relevant mutations observed in the clinic. We use mutants of a tetrapeptide sequence to probe the binding interaction, using both isothermal titration calorimetry and X-ray crystallography. Where possible, comparison between our tetrapeptide mutational study and the previously reported mutations is made, discrepancies are discussed and the importance of secondary structure in interpreting alanine scanning and mutational data of this nature is considered. | ||
| - | + | Structure-activity relationship of the peptide binding-motif mediating the BRCA2:RAD51 protein-protein interaction.,Scott DE, Marsh M, Blundell TL, Abell C, Hyvonen M FEBS Lett. 2016 Apr;590(8):1094-102. doi: 10.1002/1873-3468.12139. Epub 2016 Apr , 6. PMID:26992456<ref>PMID:26992456</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5fou" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: Abell | + | ==See Also== |
| - | [[Category: Blundell | + | *[[Resolvase 3D structures|Resolvase 3D structures]] |
| - | [[Category: Marsh | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pyrococcus furiosus]] | ||
| + | [[Category: Abell C]] | ||
| + | [[Category: Blundell TL]] | ||
| + | [[Category: Hyvonen M]] | ||
| + | [[Category: Marsh M]] | ||
| + | [[Category: Scott DE]] | ||
Current revision
HUMANISED MONOMERIC RADA IN COMPLEX WITH FHPA TETRAPEPTIDE
| |||||||||||
