Sandbox T4SS
From Proteopedia
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| - | This page is setup for Jian to build her senior project for OU CHEM 4923 | + | This page is setup for Jian to build her senior project for OU CHEM 4923 |
| - | == | + | ==FUNCTIONS OF TYPE 4 SECRETION SYSTEMS== |
| - | <StructureSection load=' | + | <StructureSection load='1gki' size='340' side='right' caption='TwrB hexamer a homologue for the VirD4 from ''E. coli''' scene=''> |
| - | + | Bacterial type IV secretions systems move DNA and proteins out of a cell. This helps bacteria give and gain DNA quickly which greatly increases the variability and therefore survivability of the bacteria. Usually transfer of this DNA/proteins requires cell-to-cell contact, however one type of T4SS releases DNA from the extracellular milieu. <ref name="one">doi:10.1111/j.1462-5822.2010.01499.x</ref> | |
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| + | ==GENERAL STRUCTURE FEATURES OF TYPE 4 SECRETION SYSTEMS== | ||
| - | - | + | With different functions comes many variations on the structure of T4SS, however the most well understood set of structures were studied through the Gram-negative bacteria ''A. tumefaciens''. It is called the VirB/VirD4 system<ref name="two">doi:10.1016/j.bbamcr.2013.12.019</ref>. |
| - | + | VirB is made up of 11 different VirB proteins systhesized from the ''virB'' operon. Most T4SS have homologes of these 12 proteins (11 from VirB and 1 from VirD) | |
| + | [[Image:Janeyuan1.1.png|thumb|Overview of T4SS structures<ref name="one" />]] | ||
| - | = | + | The diameter of the channel changes from 55 angstroms on the cytoplasmic side to 10 angstroms leading to the exterior of the cell. This indicates there are structural changes to the protein or DNA that is being transferred while in the secretion system<ref name="two" /> |
| - | == | + | ==TYPE 4 SECRETION SYSTEMS ENERGETICS== |
| - | = | + | VirB7, VirB7 and VirB10 initiate the assembly of the core of the T4SS. The complex forms spontaneously with out the input of external energy like ATP. [[Image:Janeyuan1.2.png|thumb|Overview of formation of T4SS<ref name="one" />]] |
| - | = | + | VirB4, VirB11 and VirD4 are ATPases that energizes the secretion system from the cytoplasmic side of the cell <ref name="two" />. |
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| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
This page is setup for Jian to build her senior project for OU CHEM 4923
FUNCTIONS OF TYPE 4 SECRETION SYSTEMS
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References
- ↑ 1.0 1.1 1.2 Wallden K, Rivera-Calzada A, Waksman G. Type IV secretion systems: versatility and diversity in function. Cell Microbiol. 2010 Sep 1;12(9):1203-12. doi: 10.1111/j.1462-5822.2010.01499.x. , Epub 2010 Jul 16. PMID:20642798 doi:http://dx.doi.org/10.1111/j.1462-5822.2010.01499.x
- ↑ 2.0 2.1 2.2 Christie PJ, Whitaker N, Gonzalez-Rivera C. Mechanism and structure of the bacterial type IV secretion systems. Biochim Biophys Acta. 2014 Aug;1843(8):1578-91. doi:, 10.1016/j.bbamcr.2013.12.019. Epub 2014 Jan 2. PMID:24389247 doi:http://dx.doi.org/10.1016/j.bbamcr.2013.12.019
