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5crb

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==Crystal Structure of SdeA DUB==
==Crystal Structure of SdeA DUB==
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<StructureSection load='5crb' size='340' side='right' caption='[[5crb]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='5crb' size='340' side='right'caption='[[5crb]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5crb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CRB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CRB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5crb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CRB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CRB FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5cra|5cra]], [[5crc|5crc]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5crb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5crb OCA], [http://pdbe.org/5crb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5crb RCSB], [http://www.ebi.ac.uk/pdbsum/5crb PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5crb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5crb OCA], [https://pdbe.org/5crb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5crb RCSB], [https://www.ebi.ac.uk/pdbsum/5crb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5crb ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q6RCR0_LEGPN Q6RCR0_LEGPN]
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Manipulation of the host's ubiquitin network is emerging as an important strategy for counteracting and repurposing the posttranslational modification machineries of the host by pathogens. Ubiquitin E3 ligases encoded by infectious agents are well known, as are a variety of viral deubiquitinases (DUBs). Bacterial DUBs have been discovered, but little is known about the structure and mechanism underlying their ubiquitin recognition. In this report, we found that members of the Legionella pneumophila SidE effector family harbor a DUB module important for ubiquitin dynamics on the bacterial phagosome. Structural analysis of this domain alone and in complex with ubiquitin vinyl methyl ester (Ub-VME) reveals unique molecular contacts used in ubiquitin recognition. Instead of relying on the Ile44 patch of ubiquitin, as commonly used in eukaryotic counterparts, the SdeADub module engages Gln40 of ubiquitin. The architecture of the active-site cleft presents an open arrangement with conformational plasticity, permitting deubiquitination of three of the most abundant polyubiquitin chains, with a distinct preference for Lys63 linkages. We have shown that this preference enables efficient removal of Lys63 linkages from the phagosomal surface. Remarkably, the structure reveals by far the most parsimonious use of molecular contacts to achieve deubiquitination, with less than 1,000 A2 of accessible surface area buried upon complex formation with ubiquitin. This type of molecular recognition appears to enable dual specificity toward ubiquitin and the ubiquitin-like modifier NEDD8.
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Structural basis of substrate recognition by a bacterial deubiquitinase important for dynamics of phagosome ubiquitination.,Sheedlo MJ, Qiu J, Tan Y, Paul LN, Luo ZQ, Das C Proc Natl Acad Sci U S A. 2015 Nov 23. pii: 201514568. PMID:26598703<ref>PMID:26598703</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5crb" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Das, C]]
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[[Category: Large Structures]]
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[[Category: Luo, Z Q]]
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[[Category: Legionella pneumophila]]
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[[Category: Qiu, J]]
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[[Category: Das C]]
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[[Category: Sheedlo, M J]]
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[[Category: Luo ZQ]]
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[[Category: Deubiquitinase]]
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[[Category: Qiu J]]
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[[Category: Hydrolase]]
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[[Category: Sheedlo MJ]]
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[[Category: Legionella]]
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Current revision

Crystal Structure of SdeA DUB

PDB ID 5crb

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