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3wq7

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==New crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosus==
==New crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosus==
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<StructureSection load='3wq7' size='340' side='right' caption='[[3wq7]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
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<StructureSection load='3wq7' size='340' side='right'caption='[[3wq7]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3wq7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WQ7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3wq7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WQ7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vgi|3vgi]], [[3wq0|3wq0]], [[3wq1|3wq1]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wq7 OCA], [https://pdbe.org/3wq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wq7 RCSB], [https://www.ebi.ac.uk/pdbsum/3wq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wq7 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wq7 OCA], [http://pdbe.org/3wq7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wq7 RCSB], [http://www.ebi.ac.uk/pdbsum/3wq7 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9V2T0_9EURY Q9V2T0_9EURY]
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The hyperthermophilic glycoside hydrolase family endocellulase 12 from the archaeon Pyrococcus furiosus (EGPf; Gene ID PF0854; EC 3.2.1.4) catalyzes the hydrolytic cleavage of the beta-1,4-glucosidic linkage in beta-glucan in lignocellulose biomass. A crystal of EGPf was previously prepared at pH 9.0 and its structure was determined at an atomic resolution of 1.07 A. This article reports the crystallization of EGPf at the more physiologically relevant pH of 5.5. Structure determination showed that this new crystal form has the symmetry of space group C2. Two molecules of the enzyme are observed in the asymmetric unit. Crystal packing is weak at pH 5.5 owing to two flexible interfaces between symmetry-related molecules. Comparison of the EGPf structures obtained at pH 9.0 and pH 5.5 reveals a significant conformational difference at the active centre and in the surface loops. The interfaces in the vicinity of the flexible surface loops impact the quality of the EGPf crystal.
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A new crystal form of a hyperthermophilic endocellulase.,Kataoka M, Ishikawa K Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):878-83. doi:, 10.1107/S2053230X14010930. Epub 2014 Jun 18. PMID:25005081<ref>PMID:25005081</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3wq7" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cellulase]]
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[[Category: Large Structures]]
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[[Category: Ishikawa, K]]
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[[Category: Pyrococcus furiosus]]
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[[Category: Kataoka, M]]
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[[Category: Ishikawa K]]
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[[Category: Beta-jelly roll]]
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[[Category: Kataoka M]]
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[[Category: Glycoside hydrolase]]
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[[Category: Hydrolase]]
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Current revision

New crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosus

PDB ID 3wq7

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