3jce

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'''Unreleased structure'''
 
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The entry 3jce is ON HOLD
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==Structure of Escherichia coli EF4 in pretranslocational ribosomes (Pre EF4)==
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<SX load='3jce' size='340' side='right' viewer='molstar' caption='[[3jce]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3jce]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JCE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jce OCA], [https://pdbe.org/3jce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jce RCSB], [https://www.ebi.ac.uk/pdbsum/3jce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jce ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL36_ECOLI RL36_ECOLI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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EF4 catalyzes tRNA back-translocation through an unknown mechanism. We report cryo-EM structures of Escherichia coli EF4 in post- and pretranslocational ribosomes (Post- and Pre-EF4) at 3.7- and 3.2-A resolution, respectively. In Post-EF4, peptidyl-tRNA occupies the peptidyl (P) site, but the interaction between its CCA end and the P loop is disrupted. In Pre-EF4, the peptidyl-tRNA assumes a unique position near the aminoacyl (A) site, denoted the A site/EF4 bound (A/4) site, with a large displacement at its acceptor arm. Mutagenesis analyses suggest that a specific region in the EF4 C-terminal domain (CTD) interferes with base-pairing between the peptidyl-tRNA 3'-CCA and the P loop, whereas the EF4 CTD enhances peptidyl-tRNA interaction at the A/4 site. Therefore, EF4 induces back-translocation by disengaging the tRNA's CCA end from the peptidyl transferase center of the translating ribosome.
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Authors: Zhang D, Yan K, Liu G, Song G, Luo J, Shi Y, Cheng E, Wu S, Jiang T, Low J, Gao N, Qin Y
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EF4 disengages the peptidyl-tRNA CCA end and facilitates back-translocation on the 70S ribosome.,Zhang D, Yan K, Liu G, Song G, Luo J, Shi Y, Cheng E, Wu S, Jiang T, Lou J, Gao N, Qin Y Nat Struct Mol Biol. 2016 Feb;23(2):125-31. doi: 10.1038/nsmb.3160. Epub 2016 Jan, 25. PMID:26809121<ref>PMID:26809121</ref>
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Description: Structure of EF4-bound ribosomal complexs
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Zhang D, Yan K, Liu G, Song G, Luo J, Shi Y, Cheng E, Wu S, Jiang T, Low J, Gao N, Qin Y]]
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<div class="pdbe-citations 3jce" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</SX>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Cheng E]]
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[[Category: Gao N]]
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[[Category: Jiang T]]
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[[Category: Liu G]]
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[[Category: Low J]]
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[[Category: Luo J]]
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[[Category: Qin Y]]
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[[Category: Shi Y]]
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[[Category: Song G]]
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[[Category: Wu S]]
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[[Category: Yan K]]
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[[Category: Zhang D]]

Current revision

Structure of Escherichia coli EF4 in pretranslocational ribosomes (Pre EF4)

3jce, resolution 3.20Å

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OCA

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