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Sandbox UNLPam 7

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(New page: ==Your Heading Here (maybe something like 'Structure')== <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> This is a default text for you...)
Current revision (13:45, 9 December 2015) (edit) (undo)
 
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==Your Heading Here (maybe something like 'Structure')==
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== Crystal structure of a novel two domain GH78 family a-rhamnosidase from Klebsiella oxytoca with rhamnose bound ==
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<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
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This is a default text for your page '''Sandbox UNLPam 7'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
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You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.
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== Function ==
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==Introduction==
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<StructureSection load='4xhc' size='350' side='right' caption='X-ray crystal structure of the a-L-rhamnosidase from K. oxytoca (KoRha).' scene='71/719552/4xhc_1/1'>4xhc structure</scene>'>
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== Disease ==
 
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== Relevance ==
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a-L-rhamnosidases (E.C. 3.2.1.40) are found widely distributed
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in nature and have been reported in animals,
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== Structural highlights ==
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plants, yeast, fungi and bacteria, where they are responsible
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for the cleavage of a-L-rhamnose from a wide range of
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This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
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compounds.1 a-L-rhamnose is found in plants and bacteria
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as components of polysaccharides, such as pectins,2 and
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the O antigen polysaccharides, responsible for determining
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the antigenicity of pathogenic bacteria3; it is also found in
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rhamnolipids4 and it is attached to small molecule natural
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products, such as rutin. There is industrial interest in arhamnosidases
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for use in the debittering of citrus juices
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and for the release of flavonoids from rhamnosylated precursors;
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in wine production they play a role in the hydrolysis
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of glycosylated terpene aroma compounds.
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==KoRha structure==
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The crystal structure of KoRha with rhamnose bound was
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determined to 2.7A ° resolution. The final model consisted of
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two KoRha subunits related by a non-crystallographic twofold
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axis (giving a corresponding solvent content of 73%) in
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the asymmetric unit, with each monomer containing a
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bound rhamnose. Dynamic light scattering had suggested
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that KoRha was a homodimer in solution and the structure
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of KoRha confirmed this, giving a dimer interface of 1389.9
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A °
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2 (as calculated using the PISA server (http://www.ebi.ac.
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uk/pdbe/pisa/).
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Each monomer of KoRha is composed of two
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domains. Domain A, the catalytic domain, is mainly ahelical,
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consisting of residues 11–30 and 180–523, and
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contains the bound rhamnose. Domain B, the dimerization
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domain, is a b-sandwich domain consisting of residues
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31–179.
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<scene name='71/719552/4xhc_1/1'>4xhc structure</scene>
</StructureSection>
</StructureSection>
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== References ==
 
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<references/>
 

Current revision

Crystal structure of a novel two domain GH78 family a-rhamnosidase from Klebsiella oxytoca with rhamnose bound

Introduction

X-ray crystal structure of the a-L-rhamnosidase from K. oxytoca (KoRha).

Drag the structure with the mouse to rotate
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