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1ap8

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[[Image:1ap8.gif|left|200px]]
 
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{{Structure
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==TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES==
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|PDB= 1ap8 |SIZE=350|CAPTION= <scene name='initialview01'>1ap8</scene>
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<StructureSection load='1ap8' size='340' side='right'caption='[[1ap8]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5&#39;-DIPHOSPHATE'>M7G</scene>
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<table><tr><td colspan='2'>[[1ap8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AP8 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5-DIPHOSPHATE'>M7G</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ap8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap8 OCA], [https://pdbe.org/1ap8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ap8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ap8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ap8 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap8 OCA], [http://www.ebi.ac.uk/pdbsum/1ap8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ap8 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/IF4E_YEAST IF4E_YEAST] Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ap/1ap8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ap8 ConSurf].
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<div style="clear:both"></div>
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'''TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES'''
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==See Also==
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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eIF4E, the mRNA cap binding protein, is a master switch that controls eukaryotic translation. To be active, it must bind eIF4G and form the eIF4F complex, which also contains eIF4A. Translation is downregulated by association of eIF4E with 4E-BP, which occupies the eIF4G binding site. Signalling events acting on 4E-BP cause it to dissociate from eIF4E, and eIF4E is then free to bind eIF4G to form the active eIF4F complex. We have solved the structure of the yeast eIF4E/m7Gpp complex in a CHAPS micelle. We determined the position of the second nucleotide in a complex with m7GpppA, and identified the 4E-BP binding site. eIF4E has a curved eight-stranded antiparallel beta-sheet, decorated with three helices on the convex face and three smaller helices inserted in connecting loops. The m7G of the cap is intercalated into a stack of tryptophans in the concave face. The 4E-BP binding site is located in a region encompassing one edge of the beta-sheet, the adjacent helix a2 and several regions of non-regular secondary structure. It is adjacent to, but does not overlap the cap-binding site.
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[[Category: Large Structures]]
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==About this Structure==
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1AP8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP8 OCA].
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==Reference==
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Structure of translation factor eIF4E bound to m7GDP and interaction with 4E-binding protein., Matsuo H, Li H, McGuire AM, Fletcher CM, Gingras AC, Sonenberg N, Wagner G, Nat Struct Biol. 1997 Sep;4(9):717-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9302999 9302999]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Fletcher M]]
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[[Category: Fletcher, M.]]
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[[Category: Gingras AC]]
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[[Category: Gingras, A C.]]
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[[Category: Li H]]
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[[Category: Li, H.]]
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[[Category: Matsuo H]]
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[[Category: Matsuo, H.]]
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[[Category: Mcguire AM]]
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[[Category: Mcguire, A M.]]
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[[Category: Sonenberg N]]
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[[Category: Sonenberg, N.]]
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[[Category: Wagner G]]
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[[Category: Wagner, G.]]
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[[Category: rna cap]]
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[[Category: translation initiation factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:44:53 2008''
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Current revision

TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES

PDB ID 1ap8

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