4xko

From Proteopedia

(Difference between revisions)

Current revision

N-terminal domain of Hsp90 from Dictyostelium discoideum in hexagonal form with PEG

Structural highlights

4xko is a 1 chain structure with sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.455Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HSC90_DICDI Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity).

Publication Abstract from PubMed

The involvement of Hsp90 in progression of diseases like cancer, neurological disorders and several pathogen related conditions is well established. Hsp90, therefore, has emerged as an attractive drug target for many of these diseases. Several small molecule inhibitors of Hsp90, such as geldanamycin derivatives, that display antitumor activity, have been developed and are under clinical trials. However, none of these tested inhibitors or drugs are peptide-based compounds. Here we report the first crystal structure of a peptide bound at the ATP binding site of the N-terminal domain of Hsp90. The peptide makes several specific interactions with the binding site residues, which are comparable to those made by the nucleotide and geldanamycin. A modified peptide was designed based on these interactions. Inhibition of ATPase activity of Hsp90 was observed in the presence of the modified peptide. This study provides an alternative approach and a lead peptide molecule for the rational design of effective inhibitors of Hsp90 function.

First Structural View of a Peptide Interacting with the Nucleotide Binding Domain of Heat Shock Protein 90.,Raman S, Singh M, Tatu U, Suguna K Sci Rep. 2015 Nov 24;5:17015. doi: 10.1038/srep17015. PMID:26599366^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Raman S, Singh M, Tatu U, Suguna K. First Structural View of a Peptide Interacting with the Nucleotide Binding Domain of Heat Shock Protein 90. Sci Rep. 2015 Nov 24;5:17015. doi: 10.1038/srep17015. PMID:26599366 doi:http://dx.doi.org/10.1038/srep17015

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xko"

Categories: Dictyostelium discoideum | Large Structures | Raman S | Suguna K

@@ Line 1: / Line 1: @@
 ==N-terminal domain of Hsp90 from Dictyostelium discoideum in hexagonal form with PEG==
-<StructureSection load='4xko' size='340' side='right' caption='[[4xko]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
+<StructureSection load='4xko' size='340' side='right'caption='[[4xko]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4xko]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XKO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4xko]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XKO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.455&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xko OCA], [http://pdbe.org/4xko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xko RCSB], [http://www.ebi.ac.uk/pdbsum/4xko PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xko OCA], [https://pdbe.org/4xko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xko RCSB], [https://www.ebi.ac.uk/pdbsum/4xko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xko ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/HSC90_DICDI HSC90_DICDI]] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity).
+[https://www.uniprot.org/uniprot/HSC90_DICDI HSC90_DICDI] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 4xko" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Heat Shock Protein structures|Heat Shock Protein structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Raman, S]]
+[[Category: Dictyostelium discoideum]]
-[[Category: Suguna, K]]
+[[Category: Large Structures]]
-[[Category: Chaperone]]
+[[Category: Raman S]]
-[[Category: Hsp90]]
+[[Category: Suguna K]]
-[[Category: Peg]]

4xko

From Proteopedia

Current revision

N-terminal domain of Hsp90 from Dictyostelium discoideum in hexagonal form with PEG

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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