5aa7

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==Structural and functional characterization of a chitin-active 15.5 kDa lytic polysaccharide monooxygenase domain from a modular chitinase from Jonesia denitrificans==
==Structural and functional characterization of a chitin-active 15.5 kDa lytic polysaccharide monooxygenase domain from a modular chitinase from Jonesia denitrificans==
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<StructureSection load='5aa7' size='340' side='right' caption='[[5aa7]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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<StructureSection load='5aa7' size='340' side='right'caption='[[5aa7]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5aa7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AA7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5aa7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Jonesia_denitrificans Jonesia denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AA7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa7 OCA], [http://pdbe.org/5aa7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aa7 RCSB], [http://www.ebi.ac.uk/pdbsum/5aa7 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa7 OCA], [https://pdbe.org/5aa7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aa7 RCSB], [https://www.ebi.ac.uk/pdbsum/5aa7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aa7 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/C7R4I0_JONDD C7R4I0_JONDD]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lytic polysaccharide monooxygenases (LPMOs) boost enzymatic depolymerization of recalcitrant polysaccharides, such as chitin and cellulose. We have studied a chitin-active LPMO domain (JdLPMO10A) that is considerably smaller (15.5 kDa) than all structurally characterized LPMOs so far and that is part of a modular protein containing a GH18 chitinase. The 1.55 A resolution structure revealed deletions of interacting loops that protrude from the core beta-sandwich scaffold in larger LPMO10s. Despite these deletions, the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved. JdLPMO10A may represent a minimal scaffold needed to catalyse the powerful LPMO reaction.
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Structural and functional characterization of a small chitin-active lytic polysaccharide monooxygenase domain of a multi-modular chitinase from Jonesia denitrificans.,Mekasha S, Forsberg Z, Dalhus B, Bacik JP, Choudhary S, Schmidt-Dannert C, Vaaje-Kolstad G, Eijsink VG FEBS Lett. 2016 Jan;590(1):34-42. doi: 10.1002/1873-3468.12025. Epub 2015 Dec 28. PMID:26763108<ref>PMID:26763108</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5aa7" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Chitinase 3D structures|Chitinase 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Chitinase]]
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[[Category: Jonesia denitrificans]]
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[[Category: Choudhary, S]]
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[[Category: Large Structures]]
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[[Category: Dalhus, B]]
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[[Category: Choudhary S]]
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[[Category: Eijsink, V]]
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[[Category: Dalhus B]]
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[[Category: Forsberg, Z]]
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[[Category: Eijsink V]]
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[[Category: Mekasha, S]]
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[[Category: Forsberg Z]]
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[[Category: Schmidt-Dannert, C]]
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[[Category: Mekasha S]]
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[[Category: Vaaje-Kolstad, G]]
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[[Category: Schmidt-Dannert C]]
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[[Category: Hydrolase]]
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[[Category: Vaaje-Kolstad G]]

Current revision

Structural and functional characterization of a chitin-active 15.5 kDa lytic polysaccharide monooxygenase domain from a modular chitinase from Jonesia denitrificans

PDB ID 5aa7

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