Chymotrypsin Inhibitor

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<StructureSection load='1to1' size='350' side='right' caption='Chymotrypsin Inhibitor 2 (gold) complex with subtilisin (magenta), (PDB entry [[1to1]])' scene='43/430100/Cv/2'>
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<StructureSection load='' size='350' side='right' caption='Chymotrypsin Inhibitor 2 (cyan) complex with subtilisin (magenta), PEG, citrate, Ca+2 (green) and Na+ (purple) ions (PDB entry [[1to1]])' scene='43/430100/Cv/2'>
== Function ==
== Function ==
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[[Chymotrypsin Inhibitor|Chymotrypsin Inhibitors]] (CI) are serine proteinase inhibitors of chymotrypsin. They are classified by numbers: CI-1<ref>PMID:7922044</ref>, CI-2<ref>PMID:3828302</ref>, CI-3<ref>PMID:10531477</ref>.
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[[Chymotrypsin Inhibitor|Chymotrypsin Inhibitors]] (CI) are serine proteinase inhibitors of chymotrypsin. They are classified by numbers: CI-1<ref>PMID:7922044</ref>, CI-2<ref>PMID:3828302</ref>, CI-3<ref>PMID:10531477</ref>. '''Subtilisin-chymotrypsin inhibitor''' inhibits both subtilisin and chymotrypsin.
== Structural highlights ==
== Structural highlights ==
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CI inhibition is achieved by the insertion of a loop into the serine protease active site. <ref>PMID:15504027</ref>
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CI inhibition is achieved by the <scene name='43/430100/Cv/3'>insertion of a loop into the serine protease active site</scene>. <ref>PMID:15504027</ref>
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</StructureSection>
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== 3D Structures of Chymotrypsin Inhibitor ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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== 3D Structures of Chymotrypsin inhibitor ==
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{{#tree:id=OrganizedByTopic|openlevels=0|
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[[Chymotrypsin inhibitor 3D structures]]
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* CI-1
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</StructureSection>
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**[[2jzm]] – CI-1 fragment – ''Nicotiana alata'' – NMR<br />
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**[[1eai]] – CI-1+elastase - pig<br />
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**[[1kgm]] –lCI-1 – locust - NMR<br />
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**[[1kio]] – lCI-1 (mutant) - NMR<br />
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* CI-2
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**[[1y1k]], [[1y33]], [[1y34]], [[1y3b]], [[1y3c]], [[1y3d]], [[1y3f]], [[1y48]], [[1y4a]], [[1y4d]], [[1tm1]], [[1tm3]], [[1tm4]], [[1tm5]], [[1tm7]], [[1tmg]], [[1to1]], [[1to2]], [[1lw6]] – BaCI-2 (mutant)+subtilisin – ''Bacillus amyloliquefaciens''<br />
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**[[2sni]] - BaCI-2+subtilisin<br />
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**[[1ciq]], [[1cir]] – HvCI-2 fragments – ''Hordeum vulgare''<br />
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**[[1coa]], [[1ypa]], [[1ypb]], [[1ypc]] – HvCI-2 (mutant)<br />
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**[[1cis]] - HvCI-2+subtilisin helix – NMR<br />
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**[[3ci2]] - HvCI-2 – NMR<br />
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**[[2ci2]] - HvCI-2<br />
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**[[1cq4]] - HvCI-2 domain swap
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* CI-3
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**[[3i2a]], [[3i2x]], [[2bea]], [[2beb]], [[2esu]], [[2et2]], [[1xg6]], [[1fmz]], [[1fn0]], [[1eyl]], [[3qyd]], [[4h9w]], [[4ha2]], [[4tlp]] – PtCI-3 (mutant) – ''Phosphocarpus tetragonolobus''<br />
 
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**[[4wbc]], [[2wbc]], [[1wbc]] - PtCI-3<br />
 
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**[[3i29]], [[3veq]] - PtCI-3 (mutant)+trypsin<br />
 
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**[[2qyi]] – CI-3 (mutant)+cationic trypsin – cow<br />
 
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**[[1ccv]] – CI –''Apis mellifera'' - NMR<br />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Chymotrypsin Inhibitor 2 (cyan) complex with subtilisin (magenta), PEG, citrate, Ca+2 (green) and Na+ (purple) ions (PDB entry 1to1)

Drag the structure with the mouse to rotate

References

  1. ↑ Huang K, Strynadka NC, Bernard VD, Peanasky RJ, James MN. The molecular structure of the complex of Ascaris chymotrypsin/elastase inhibitor with porcine elastase. Structure. 1994 Jul 15;2(7):679-89. PMID:7922044
  2. ↑ McPhalen CA, James MN. Crystal and molecular structure of the serine proteinase inhibitor CI-2 from barley seeds. Biochemistry. 1987 Jan 13;26(1):261-9. PMID:3828302
  3. ↑ Ravichandran S, Sen U, Chakrabarti C, Dattagupta JK. Cryocrystallography of a Kunitz-type serine protease inhibitor: the 90 K structure of winged bean chymotrypsin inhibitor (WCI) at 2.13 A resolution. Acta Crystallogr D Biol Crystallogr. 1999 Nov;55(Pt 11):1814-21. PMID:10531477
  4. ↑ Radisky ES, Kwan G, Karen Lu CJ, Koshland DE Jr. Binding, proteolytic, and crystallographic analyses of mutations at the protease-inhibitor interface of the subtilisin BPN'/chymotrypsin inhibitor 2 complex. Biochemistry. 2004 Nov 2;43(43):13648-56. PMID:15504027 doi:http://dx.doi.org/10.1021/bi048797k

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