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3jc5
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the eukaryotic replicative CMG helicase and pumpjack motion== | |
| - | + | <SX load='3jc5' size='340' side='right' viewer='molstar' caption='[[3jc5]], [[Resolution|resolution]] 4.70Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3jc5]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JC5 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.7Å</td></tr> | |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jc5 OCA], [https://pdbe.org/3jc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jc5 RCSB], [https://www.ebi.ac.uk/pdbsum/3jc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jc5 ProSAT]</span></td></tr> |
| - | [[Category: | + | </table> |
| - | [[Category: | + | == Function == |
| - | [[Category: | + | [https://www.uniprot.org/uniprot/MCM2_YEAST MCM2_YEAST] Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Necessary for cell growth.<ref>PMID:19896182</ref> <ref>PMID:19910535</ref> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </SX> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae]] | ||
| + | [[Category: Bai L]] | ||
| + | [[Category: Georgescu RE]] | ||
| + | [[Category: Li H]] | ||
| + | [[Category: Liu J]] | ||
| + | [[Category: O'Donnell ME]] | ||
| + | [[Category: Sun J]] | ||
| + | [[Category: Yuan Z]] | ||
Current revision
Structure of the eukaryotic replicative CMG helicase and pumpjack motion
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Categories: Large Structures | Saccharomyces cerevisiae | Bai L | Georgescu RE | Li H | Liu J | O'Donnell ME | Sun J | Yuan Z
