5ce8

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'''Unreleased structure'''
 
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The entry 5ce8 is ON HOLD
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==Crystal structure of branched-chain aminotransferase from thermophilic archaea Thermoproteus uzoniensis==
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<StructureSection load='5ce8' size='340' side='right'caption='[[5ce8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5ce8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoproteus_uzoniensis_768-20 Thermoproteus uzoniensis 768-20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CE8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ce8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ce8 OCA], [https://pdbe.org/5ce8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ce8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ce8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ce8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/F2L0W0_THEU7 F2L0W0_THEU7]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The gene TUZN1299 from the genome of the hyperthermophilic archaeon Thermoproteus uzoniensis encoding a new 32.8 kDa branched-chain amino acid aminotransferase (BCAT) was expressed in Escherichia coli. The recombinant protein TUZN1299 was purified to homogeneity in the PLP-bound form. TUZN1299 was active towards branched-chain amino acids (L-Val, L-Leu, L-Ile) and showed low but detectable activity toward (R)-alpha-methylbenzylamine. The enzyme exhibits high-temperature optimum, thermal stability, and tolerance to organic solvents. The structure of an archaeal BCAT called TUZN1299 was solved for the first time (at 2.0 A resolution). TUZN1299 has a typical BCAT type IV fold, and the organization of its active site is similar to that of bacterial BCATs. However, there are some differences in the amino acid composition of the active site.
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Authors: Boyko, K.M., Nikolaeva, A.Y., Stekhanova, T.N., Mardanov, A.V., Rakitin, A.L., Ravin, N.V., Popov, V.O.
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First structure of archaeal branched-chain amino acid aminotransferase from Thermoproteus uzoniensis specific for L-amino acids and R-amines.,Boyko KM, Stekhanova TN, Nikolaeva AY, Mardanov AV, Rakitin AL, Ravin NV, Bezsudnova EY, Popov VO Extremophiles. 2016 Mar;20(2):215-25. doi: 10.1007/s00792-016-0816-z. Epub 2016, Feb 12. PMID:26872794<ref>PMID:26872794</ref>
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Description: Crystal structure of branched-chain aminotransferase from thermophilic archaea Thermoproteus uzoniensis
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Mardanov, A.V]]
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<div class="pdbe-citations 5ce8" style="background-color:#fffaf0;"></div>
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[[Category: Ravin, N.V]]
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[[Category: Boyko, K.M]]
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==See Also==
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[[Category: Popov, V.O]]
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*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
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[[Category: Stekhanova, T.N]]
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== References ==
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[[Category: Nikolaeva, A.Y]]
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<references/>
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[[Category: Rakitin, A.L]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermoproteus uzoniensis 768-20]]
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[[Category: Boyko KM]]
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[[Category: Mardanov AV]]
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[[Category: Nikolaeva AY]]
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[[Category: Popov VO]]
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[[Category: Rakitin AL]]
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[[Category: Ravin NV]]
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[[Category: Stekhanova TN]]

Current revision

Crystal structure of branched-chain aminotransferase from thermophilic archaea Thermoproteus uzoniensis

PDB ID 5ce8

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