5bwy

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==Structure of proplasmepsin II from Plasmodium falciparum, Space Group P43212==
==Structure of proplasmepsin II from Plasmodium falciparum, Space Group P43212==
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<StructureSection load='5bwy' size='340' side='right' caption='[[5bwy]], [[Resolution|resolution]] 2.64&Aring;' scene=''>
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<StructureSection load='5bwy' size='340' side='right'caption='[[5bwy]], [[Resolution|resolution]] 2.64&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5bwy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BWY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BWY FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5bwy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BWY FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bwy OCA], [http://pdbe.org/5bwy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bwy RCSB], [http://www.ebi.ac.uk/pdbsum/5bwy PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.644&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bwy OCA], [https://pdbe.org/5bwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bwy RCSB], [https://www.ebi.ac.uk/pdbsum/5bwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bwy ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLM2_PLAFX PLM2_PLAFX] During the asexual blood stage, participates in initial cleavage of native host hemoglobin (Hb) resulting in Hb denaturation (PubMed:11782538, PubMed:15574427, PubMed:8844673). May cleave preferentially denatured hemoglobin that has been cleaved by PMI (PubMed:8844673). Digestion of host Hb is an essential step which provides the parasite with amino acids for protein synthesis, and regulates osmolarity (Probable).<ref>PMID:11782538</ref> <ref>PMID:15574427</ref> <ref>PMID:8844673</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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Proplasmepsin II is the zymogen of plasmepsin II, an aspartic proteinase used by Plasmodiumfalciparum to digest hemoglobin during the blood stage of malaria. A large shift between the N-domain and the central and C-domains of proplasmepsin II opens the active site cleft, preventing the formation of a functional aspartic proteinase active site. This mode of inhibition of catalytic activity has not been observed in any other aspartic proteinase zymogen. Instead of occluding a pre-formed active site, as in the gastric aspartic proteinase zymogens, the prosegment of proplasmepsin II interacts extensively with the C-domain and serves as a 'harness' to keep the domains apart. Disruption of key salt bridges at low pH may be important in activation.
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Plasmepsin IV from Plasmodium falciparum (PM IV) is a promising target for the development of novel antimalarial drugs. Here, the crystal structure of the truncated zymogen of PM IV (pPM IV), consisting of the mature enzyme plus a prosegment of 47 residues, has been determined at 1.5 A resolution. pPM IV presents the fold previously described for studied proplasmepsins, displaying closer similarities to proplasmepin IV from P. vivax (pPvPM) than to the other two proplasmepsins from P. falciparum. The study and comparison of the pPM IV structure with the proplasmepsin structures described previously provide information about the similarities and differences in the inactivation-activation mechanisms among the plasmepsin zymogens.
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Crystal structure of the novel aspartic proteinase zymogen proplasmepsin II from plasmodium falciparum.,Bernstein NK, Cherney MM, Loetscher H, Ridley RG, James MN Nat Struct Biol. 1999 Jan;6(1):32-7. PMID:9886289<ref>PMID:9886289</ref>
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Crystal structure of Plasmodium falciparum proplasmepsin IV: the plasticity of proplasmepsins.,Recacha R, Jaudzems K, Akopjana I, Jirgensons A, Tars K Acta Crystallogr F Struct Biol Commun. 2016 Sep;72(Pt 9):659-66. doi:, 10.1107/S2053230X16011663. Epub 2016 Aug 9. PMID:27599854<ref>PMID:27599854</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 5bwy" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 5bwy" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Plasmepsin|Plasmepsin]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Akopjana, I]]
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[[Category: Large Structures]]
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[[Category: Jaudzems, K]]
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[[Category: Plasmodium falciparum]]
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[[Category: Recacha, R]]
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[[Category: Akopjana I]]
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[[Category: Tars, K]]
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[[Category: Jaudzems K]]
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[[Category: Hydrolase]]
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[[Category: Recacha R]]
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[[Category: Malaria]]
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[[Category: Tars K]]

Current revision

Structure of proplasmepsin II from Plasmodium falciparum, Space Group P43212

PDB ID 5bwy

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