1b69

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[[Image:1b69.gif|left|200px]]
 
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{{Structure
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==THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX==
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|PDB= 1b69 |SIZE=350|CAPTION= <scene name='initialview01'>1b69</scene>
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<StructureSection load='1b69' size='340' side='right'caption='[[1b69]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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<table><tr><td colspan='2'>[[1b69]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B69 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b69 OCA], [https://pdbe.org/1b69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b69 RCSB], [https://www.ebi.ac.uk/pdbsum/1b69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b69 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1tn9|1TN9]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b69 OCA], [http://www.ebi.ac.uk/pdbsum/1b69 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b69 RCSB]</span>
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[https://www.uniprot.org/uniprot/TNR6_ENTFL TNR6_ENTFL]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/1b69_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b69 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The integrase protein catalyzes the excision and integration of the Tn916 conjugative transposon, a promiscuous genetic element that spreads antibiotic resistance in pathogenic bacteria. The solution structure of the N-terminal domain of the Tn916 integrase protein bound to its DNA-binding site within the transposon arm has been determined. The structure reveals an interesting mode of DNA recognition, in which the face of a three-stranded antiparallel beta-sheet is positioned within the major groove. A comparison to the structure of the homing endonuclease I-Ppol-DNA complex suggests that the three-stranded sheet may represent a new DNA-binding motif whose residue composition and position within the major groove are varied to alter specificity. The structure also provides insights into the mechanism of conjugative transposition. The DNA in the complex is bent approximately 35 degrees and may, together with potential interactions between bound integrase proteins at directly repeated sites, significantly bend the arms of the transposon.
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'''THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX'''
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NMR structure of the Tn916 integrase-DNA complex.,Wojciak JM, Connolly KM, Clubb RT Nat Struct Biol. 1999 Apr;6(4):366-73. PMID:10201406<ref>PMID:10201406</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1b69" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The integrase protein catalyzes the excision and integration of the Tn916 conjugative transposon, a promiscuous genetic element that spreads antibiotic resistance in pathogenic bacteria. The solution structure of the N-terminal domain of the Tn916 integrase protein bound to its DNA-binding site within the transposon arm has been determined. The structure reveals an interesting mode of DNA recognition, in which the face of a three-stranded antiparallel beta-sheet is positioned within the major groove. A comparison to the structure of the homing endonuclease I-Ppol-DNA complex suggests that the three-stranded sheet may represent a new DNA-binding motif whose residue composition and position within the major groove are varied to alter specificity. The structure also provides insights into the mechanism of conjugative transposition. The DNA in the complex is bent approximately 35 degrees and may, together with potential interactions between bound integrase proteins at directly repeated sites, significantly bend the arms of the transposon.
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*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1B69 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B69 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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NMR structure of the Tn916 integrase-DNA complex., Wojciak JM, Connolly KM, Clubb RT, Nat Struct Biol. 1999 Apr;6(4):366-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10201406 10201406]
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[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Clubb, R T.]]
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[[Category: Synthetic construct]]
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[[Category: Connolly, K M.]]
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[[Category: Clubb RT]]
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[[Category: Wojciak, J M.]]
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[[Category: Connolly KM]]
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[[Category: beta-sheet recognition]]
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[[Category: Wojciak JM]]
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[[Category: complex]]
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[[Category: dna binding]]
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[[Category: integrase]]
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[[Category: transposition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:54:31 2008''
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Current revision

THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX

PDB ID 1b69

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