1b73

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (23:20, 27 December 2023) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1b73.jpg|left|200px]]
 
-
{{Structure
+
==GLUTAMATE RACEMASE FROM AQUIFEX PYROPHILUS==
-
|PDB= 1b73 |SIZE=350|CAPTION= <scene name='initialview01'>1b73</scene>, resolution 2.3&Aring;
+
<StructureSection load='1b73' size='340' side='right'caption='[[1b73]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1b73]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_pyrophilus Aquifex pyrophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B73 FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_racemase Glutamate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.3 5.1.1.3] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b73 OCA], [https://pdbe.org/1b73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b73 RCSB], [https://www.ebi.ac.uk/pdbsum/1b73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b73 ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=[[1b74|1B74]]
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b73 OCA], [http://www.ebi.ac.uk/pdbsum/1b73 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b73 RCSB]</span>
+
[https://www.uniprot.org/uniprot/MURI_AQUPY MURI_AQUPY] Provides the (R)-glutamate required for cell wall biosynthesis. Converts L- or D-glutamate to D- or L-glutamate, respectively, but not other amino acids such as alanine, aspartate, and glutamine.
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/1b73_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b73 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Glutamate racemase (MurI) is responsible for the synthesis of D-glutamate, an essential building block of the peptidoglycan layer in bacterial cell walls. The crystal structure of glutamate racemase from Aquifex pyrophilus, determined at 2.3 A resolution, reveals that the enzyme forms a dimer and each monomer consists of two alpha/beta fold domains, a unique structure that has not been observed in other racemases or members of an enolase superfamily. A substrate analog, D-glutamine, binds to the deep pocket formed by conserved residues from two monomers. The structural and mutational analyses allow us to propose a mechanism of metal cofactor-independent glutamate racemase in which two cysteine residues are involved in catalysis.
-
'''GLUTAMATE RACEMASE FROM AQUIFEX PYROPHILUS'''
+
Structure and mechanism of glutamate racemase from Aquifex pyrophilus.,Hwang KY, Cho CS, Kim SS, Sung HC, Yu YG, Cho Y Nat Struct Biol. 1999 May;6(5):422-6. PMID:10331867<ref>PMID:10331867</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1b73" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
Glutamate racemase (MurI) is responsible for the synthesis of D-glutamate, an essential building block of the peptidoglycan layer in bacterial cell walls. The crystal structure of glutamate racemase from Aquifex pyrophilus, determined at 2.3 A resolution, reveals that the enzyme forms a dimer and each monomer consists of two alpha/beta fold domains, a unique structure that has not been observed in other racemases or members of an enolase superfamily. A substrate analog, D-glutamine, binds to the deep pocket formed by conserved residues from two monomers. The structural and mutational analyses allow us to propose a mechanism of metal cofactor-independent glutamate racemase in which two cysteine residues are involved in catalysis.
+
*[[Glutamate racemase 3D structures|Glutamate racemase 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1B73 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_pyrophilus Aquifex pyrophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B73 OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
Structure and mechanism of glutamate racemase from Aquifex pyrophilus., Hwang KY, Cho CS, Kim SS, Sung HC, Yu YG, Cho Y, Nat Struct Biol. 1999 May;6(5):422-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10331867 10331867]
+
[[Category: Aquifex pyrophilus]]
[[Category: Aquifex pyrophilus]]
-
[[Category: Glutamate racemase]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Cho CS]]
-
[[Category: Cho, C S.]]
+
[[Category: Cho Y]]
-
[[Category: Cho, Y.]]
+
[[Category: Hwang KY]]
-
[[Category: Hwang, K Y.]]
+
[[Category: Kim SS]]
-
[[Category: Kim, S S.]]
+
[[Category: Yu YG]]
-
[[Category: Yu, Y G.]]
+
-
[[Category: isomerase]]
+
-
[[Category: racemase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:54:59 2008''
+

Current revision

GLUTAMATE RACEMASE FROM AQUIFEX PYROPHILUS

PDB ID 1b73

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1b73"
Personal tools