5h9t

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'''Unreleased structure'''
 
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The entry 5h9t is ON HOLD
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==Crystal structure of native NalD at resolution of 2.9, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa==
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<StructureSection load='5h9t' size='340' side='right'caption='[[5h9t]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5h9t]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H9T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H9T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.89&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h9t OCA], [https://pdbe.org/5h9t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h9t RCSB], [https://www.ebi.ac.uk/pdbsum/5h9t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h9t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9HY46_PSEAE Q9HY46_PSEAE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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NalD was reported to be the secondary repressor of the MexAB-OprM multidrug efflux pump, the major system contributing to intrinsic multidrug resistance in Pseudomonas aeruginosa. Here, we show that novobiocin binds directly to NalD, which leads NalD to dissociate from the DNA promoter, and thus de-represses the expression of the MexAB-OprM pump. In addition, we have solved the crystal structure of NalD at a resolution of 2.90 A. The structural alignment of NalD to its homologue TtgR reveals that the residues N129 and H167 in NalD are involved in its novobiocin-binding ability. We have confirmed the function of these two amino acids by EMSA and plate assay. The results presented here highlight the importance and diversity of regulatory mechanism in bacterial antibiotic resistance, and provide further insight for novel antimicrobial development. This article is protected by copyright. All rights reserved.
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Authors: Chen, W.Z., Wang, D., Huang, S.Q., Hu, Q.Y., Liu, X.C., Gan, J.H., Chen, H.
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Novobiocin binding to NalD induces the expression of the MexAB-OprM pump in Pseudomonas aeruginosa.,Chen W, Wang D, Zhou W, Sang H, Liu X, Ge Z, Zhang J, Lan L, Yang CG, Chen H Mol Microbiol. 2016 Feb 4. doi: 10.1111/mmi.13346. PMID:26844397<ref>PMID:26844397</ref>
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Description: Crystal structure of native NalD at resolution of 2.9, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Hu, Q.Y]]
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<div class="pdbe-citations 5h9t" style="background-color:#fffaf0;"></div>
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[[Category: Wang, D]]
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== References ==
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[[Category: Liu, X.C]]
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<references/>
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[[Category: Chen, H]]
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__TOC__
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[[Category: Gan, J.H]]
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</StructureSection>
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[[Category: Chen, W.Z]]
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[[Category: Large Structures]]
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[[Category: Huang, S.Q]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Chen H]]
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[[Category: Chen WZ]]
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[[Category: Gan JH]]
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[[Category: Hu QY]]
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[[Category: Huang SQ]]
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[[Category: Liu XC]]
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[[Category: Wang D]]

Current revision

Crystal structure of native NalD at resolution of 2.9, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa

PDB ID 5h9t

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