1bf4

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[[Image:1bf4.gif|left|200px]]
 
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{{Structure
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==CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX==
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|PDB= 1bf4 |SIZE=350|CAPTION= <scene name='initialview01'>1bf4</scene>, resolution 1.6&Aring;
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<StructureSection load='1bf4' size='340' side='right'caption='[[1bf4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=5IU:5-IODO-2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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<table><tr><td colspan='2'>[[1bf4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BF4 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bf4 OCA], [https://pdbe.org/1bf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bf4 RCSB], [https://www.ebi.ac.uk/pdbsum/1bf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bf4 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bf4 OCA], [http://www.ebi.ac.uk/pdbsum/1bf4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bf4 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/DN7D_SULAC DN7D_SULAC] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990]
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__TOC__
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'''CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX'''
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus solfataricus and S. acidocaldarius respectively. These proteins have high thermal, acid and chemical stability. They bind DNA without marked sequence preference and increase the Tm of DNA by approximately 40 degrees C. Sso7d in complex with GTAATTAC and GCGT(iU)CGC + GCGAACGC was crystallized in different crystal lattices and the crystal structures were solved at high resolution. Sso7d binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 60 degrees) by the intercalation of the hydrophobic side chains of Val 26 and Met 29. The intercalation sites are different in the two complexes. Observations of this novel DNA binding mode in three independent crystal lattices indicate that it is not a function of crystal packing.
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==About this Structure==
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1BF4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BF4 OCA].
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==Reference==
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The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA., Gao YG, Su SY, Robinson H, Padmanabhan S, Lim L, McCrary BS, Edmondson SP, Shriver JW, Wang AH, Nat Struct Biol. 1998 Sep;5(9):782-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9731772 9731772]
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[[Category: Single protein]]
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[[Category: Sulfolobus acidocaldarius]]
[[Category: Sulfolobus acidocaldarius]]
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[[Category: Gao, Y G.]]
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[[Category: Gao Y-G]]
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[[Category: Lim, L.]]
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[[Category: Lim L]]
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[[Category: Padmanabhan, S.]]
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[[Category: Padmanabhan S]]
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[[Category: Robinson, H.]]
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[[Category: Robinson H]]
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[[Category: Shriver, J W.]]
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[[Category: Shriver JW]]
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[[Category: Su, S.]]
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[[Category: Su S]]
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[[Category: Wang, A H.J.]]
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[[Category: Wang AH-J]]
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[[Category: archaebacteria]]
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[[Category: complex (dna-binding protein/dna)]]
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[[Category: dna binding protein]]
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[[Category: hyperthermophile]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:59:47 2008''
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Current revision

CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX

PDB ID 1bf4

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