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| | ==Superoxide reductase (Neelaredoxin) from Archaeoglobus fulgidus== | | ==Superoxide reductase (Neelaredoxin) from Archaeoglobus fulgidus== |
| - | <StructureSection load='4bgl' size='340' side='right' caption='[[4bgl]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='4bgl' size='340' side='right'caption='[[4bgl]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4bgl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BGL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4bgl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BGL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgl OCA], [http://pdbe.org/4bgl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bgl RCSB], [http://www.ebi.ac.uk/pdbsum/4bgl PDBsum]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgl OCA], [https://pdbe.org/4bgl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bgl RCSB], [https://www.ebi.ac.uk/pdbsum/4bgl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bgl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SOR_ARCFU SOR_ARCFU]] Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide (By similarity). | + | [https://www.uniprot.org/uniprot/SOR_ARCFU SOR_ARCFU] Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arcfl]] | + | [[Category: Archaeoglobus fulgidus]] |
| - | [[Category: Bandeiras, T M]] | + | [[Category: Large Structures]] |
| - | [[Category: Barradas, A R]] | + | [[Category: Bandeiras TM]] |
| - | [[Category: Matias, P M]] | + | [[Category: Barradas AR]] |
| - | [[Category: Pinho, F G]] | + | [[Category: Matias PM]] |
| - | [[Category: Pinto, A F]] | + | [[Category: Pinho FG]] |
| - | [[Category: Rodrigues, J V]] | + | [[Category: Pinto AF]] |
| - | [[Category: Romao, C V]] | + | [[Category: Rodrigues JV]] |
| - | [[Category: Sousa, C M]] | + | [[Category: Romao CV]] |
| - | [[Category: Teixeira, M]] | + | [[Category: Sousa CM]] |
| - | [[Category: Oxidoreductase]]
| + | [[Category: Teixeira M]] |
| - | [[Category: Oxygen detoxification]]
| + | |
| Structural highlights
Function
SOR_ARCFU Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide (By similarity).
Publication Abstract from PubMed
Superoxide reductase (SOR), which is commonly found in prokaryotic organisms, affords protection from oxidative stress by reducing the superoxide anion to hydrogen peroxide. The reaction is catalyzed at the iron centre, which is highly conserved among the prokaryotic SORs structurally characterized to date. Reported here is the first structure of an SOR from a eukaryotic organism, the protozoan parasite Giardia intestinalis (GiSOR), which was solved at 2.0 A resolution. By collecting several diffraction data sets at 100 K from the same flash-cooled protein crystal using synchrotron X-ray radiation, photoreduction of the iron centre was observed. Reduction was monitored using an online UV-visible microspectrophotometer, following the decay of the 647 nm absorption band characteristic of the iron site in the glutamate-bound, oxidized state. Similarly to other 1Fe-SORs structurally characterized to date, the enzyme displays a tetrameric quaternary-structure arrangement. As a distinctive feature, the N-terminal loop of the protein, containing the characteristic EKHxP motif, revealed an unusually high flexibility regardless of the iron redox state. At variance with previous evidence collected by X-ray crystallography and Fourier transform infrared spectroscopy of prokaryotic SORs, iron reduction did not lead to dissociation of glutamate from the catalytic metal or other structural changes; however, the glutamate ligand underwent X-ray-induced chemical changes, revealing high sensitivity of the GiSOR active site to X-ray radiation damage.
Superoxide reductase from Giardia intestinalis: structural characterization of the first SOR from a eukaryotic organism shows an iron centre that is highly sensitive to photoreduction.,Sousa CM, Carpentier P, Matias PM, Testa F, Pinho F, Sarti P, Giuffre A, Bandeiras TM, Romao CV Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2236-47. doi:, 10.1107/S1399004715015825. Epub 2015 Oct 31. PMID:26527141[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sousa CM, Carpentier P, Matias PM, Testa F, Pinho F, Sarti P, Giuffre A, Bandeiras TM, Romao CV. Superoxide reductase from Giardia intestinalis: structural characterization of the first SOR from a eukaryotic organism shows an iron centre that is highly sensitive to photoreduction. Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2236-47. doi:, 10.1107/S1399004715015825. Epub 2015 Oct 31. PMID:26527141 doi:http://dx.doi.org/10.1107/S1399004715015825
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