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| | ==Spliceosomal U4 snRNP core domain== | | ==Spliceosomal U4 snRNP core domain== |
| - | <StructureSection load='4wzj' size='340' side='right' caption='[[4wzj]], [[Resolution|resolution]] 3.60Å' scene=''> | + | <StructureSection load='4wzj' size='340' side='right'caption='[[4wzj]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wzj]] is a 96 chain structure. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4v5u 4v5u], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y9a 2y9a], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y9b 2y9b], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y9c 2y9c] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y9d 2y9d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WZJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wzj]] is a 96 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4v5u 4v5u], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y9a 2y9a], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y9b 2y9b], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y9c 2y9c] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y9d 2y9d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WZJ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4v5u|4v5u]], [[4pjo|4pjo]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wzj OCA], [http://pdbe.org/4wzj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wzj RCSB], [http://www.ebi.ac.uk/pdbsum/4wzj PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wzj OCA], [https://pdbe.org/4wzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wzj RCSB], [https://www.ebi.ac.uk/pdbsum/4wzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wzj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | {{Large structure}} | |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RUXE_HUMAN RUXE_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/SMD1_HUMAN SMD1_HUMAN]] May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. [[http://www.uniprot.org/uniprot/RUXG_HUMAN RUXG_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/RSMB_HUMAN RSMB_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. May have a functional role in the pre-mRNA splicing or in snRNP structure. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner (By similarity). [[http://www.uniprot.org/uniprot/SMD3_HUMAN SMD3_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.<ref>PMID:11574479</ref> [[http://www.uniprot.org/uniprot/SMD2_HUMAN SMD2_HUMAN]] Required for pre-mRNA splicing. Required for snRNP biogenesis (By similarity). [[http://www.uniprot.org/uniprot/RUXF_HUMAN RUXF_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. | + | [https://www.uniprot.org/uniprot/SMD3_HUMAN SMD3_HUMAN] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.<ref>PMID:11574479</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4wzj" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4wzj" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] |
| | + | *[[Sm-like protein 3D structures|Sm-like protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Leung, A K.W]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Li, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Nagai, K]] | + | [[Category: Leung AKW]] |
| - | [[Category: Pre-mrna splicing]] | + | [[Category: Li J]] |
| - | [[Category: Sm site binding]] | + | [[Category: Nagai K]] |
| - | [[Category: Snrnp biogenesis]]
| + | |
| - | [[Category: Splicing]]
| + | |
| - | [[Category: Splicing-rna complex]]
| + | |
| Structural highlights
Function
SMD3_HUMAN Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.[1]
Publication Abstract from PubMed
The spliceosome is a dynamic macromolecular machine that assembles on pre-messenger RNA substrates and catalyses the excision of non-coding intervening sequences (introns). Four of the five major components of the spliceosome, U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), contain seven Sm proteins (SmB/B', SmD1, SmD2, SmD3, SmE, SmF and SmG) in common. Following export of the U1, U2, U4 and U5 snRNAs to the cytoplasm, the seven Sm proteins, chaperoned by the survival of motor neurons (SMN) complex, assemble around a single-stranded, U-rich sequence called the Sm site in each small nuclear RNA (snRNA), to form the core domain of the respective snRNP particle. Core domain formation is a prerequisite for re-import into the nucleus, where these snRNPs mature via addition of their particle-specific proteins. Here we present a crystal structure of the U4 snRNP core domain at 3.6 A resolution, detailing how the Sm site heptad (AUUUUUG) binds inside the central hole of the heptameric ring of Sm proteins, interacting one-to-one with SmE-SmG-SmD3-SmB-SmD1-SmD2-SmF. An irregular backbone conformation of the Sm site sequence combined with the asymmetric structure of the heteromeric protein ring allows each base to interact in a distinct manner with four key residues at equivalent positions in the L3 and L5 loops of the Sm fold. A comparison of this structure with the U1 snRNP at 5.5 A resolution reveals snRNA-dependent structural changes outside the Sm fold, which may facilitate the binding of particle-specific proteins that are crucial to biogenesis of spliceosomal snRNPs.
Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis.,Leung AK, Nagai K, Li J Nature. 2011 Apr 24. PMID:21516107[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pillai RS, Will CL, Luhrmann R, Schumperli D, Muller B. Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein. EMBO J. 2001 Oct 1;20(19):5470-9. PMID:11574479 doi:10.1093/emboj/20.19.5470
- ↑ Leung AK, Nagai K, Li J. Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis. Nature. 2011 Apr 24. PMID:21516107 doi:10.1038/nature09956
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