5hc9

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'''Unreleased structure'''
 
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The entry 5hc9 is ON HOLD
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==Thermotoga maritima CCA-adding enzyme complexed with tRNA_CCA==
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<StructureSection load='5hc9' size='340' side='right'caption='[[5hc9]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5hc9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] and [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HC9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hc9 OCA], [https://pdbe.org/5hc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hc9 RCSB], [https://www.ebi.ac.uk/pdbsum/5hc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hc9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9WZH4_THEMA Q9WZH4_THEMA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CCA-adding enzyme adds the 3'-CCA of tRNA, using CTP and ATP as substrates, and terminates RNA synthesis after completion of CCA addition, without using a nucleic acid template. The complex structure of class-II Thermotoga maritima CCA-adding enzyme and mature tRNA with 3'-CCA revealed the mechanisms by which the enzyme terminates RNA synthesis after completion of 3'-CCA addition, and discriminates 3'-mature tRNA from 3'-immature tRNA. After completion of 3'-CCA addition at the catalytic site, the 3'-CCA refolds and relocates to the release site, which is discrete from the catalytic site. The 3'-CCA forms a continuously stacked, stable conformation together with the enzyme. Consequently, the 3'-mature tRNA rotates relative to the surface of the enzyme, and only the 3'-mature tRNA is ready for release. The 3'-regions of immature tRNAs cannot form the stable stacking conformation in the release site; thus, the 3' end is relocated in the catalytic site, and the 3'-CCA is reconstructed.
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Authors: Yamashita, S., Tomita, K.
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Mechanism of 3'-Matured tRNA Discrimination from 3'-Immature tRNA by Class-II CCA-Adding Enzyme.,Yamashita S, Tomita K Structure. 2016 Jun 7;24(6):918-25. doi: 10.1016/j.str.2016.03.022. Epub 2016 Apr, 28. PMID:27133023<ref>PMID:27133023</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Yamashita, S]]
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<div class="pdbe-citations 5hc9" style="background-color:#fffaf0;"></div>
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[[Category: Tomita, K]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermotoga maritima]]
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[[Category: Thermotoga maritima MSB8]]
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[[Category: Tomita K]]
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[[Category: Yamashita S]]

Current revision

Thermotoga maritima CCA-adding enzyme complexed with tRNA_CCA

PDB ID 5hc9

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