5hd2
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The crystal structure of SeMet-Cry51Aa2-L11M== |
| + | <StructureSection load='5hd2' size='340' side='right'caption='[[5hd2]], [[Resolution|resolution]] 2.28Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hd2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HD2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.276Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hd2 OCA], [https://pdbe.org/5hd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hd2 RCSB], [https://www.ebi.ac.uk/pdbsum/5hd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hd2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/E9KBU4_BACTU E9KBU4_BACTU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts. | ||
| - | + | A transgenic approach for controlling Lygus in cotton.,Gowda A, Rydel TJ, Wollacott AM, Brown RS, Akbar W, Clark TL, Flasinski S, Nageotte JR, Read AC, Shi X, Werner BJ, Pleau MJ, Baum JA Nat Commun. 2016 Jul 18;7:12213. doi: 10.1038/ncomms12213. PMID:27426014<ref>PMID:27426014</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Rydel | + | <div class="pdbe-citations 5hd2" style="background-color:#fffaf0;"></div> |
| - | [[Category: Sturman | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus thuringiensis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Moshiri F]] | ||
| + | [[Category: Rydel TJ]] | ||
| + | [[Category: Sturman EJ]] | ||
Current revision
The crystal structure of SeMet-Cry51Aa2-L11M
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