1bkj

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[[Image:1bkj.gif|left|200px]]
 
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{{Structure
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==NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI==
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|PDB= 1bkj |SIZE=350|CAPTION= <scene name='initialview01'>1bkj</scene>, resolution 1.8&Aring;
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<StructureSection load='1bkj' size='340' side='right'caption='[[1bkj]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE= <scene name='pdbsite=ASA:Fmn+Cofactor+Binding+Site-Chain+A'>ASA</scene> and <scene name='pdbsite=ASB:Fmn+Cofactor+Binding+Site-Chain+B'>ASB</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1bkj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cum 1cum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BKJ FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase FMN reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.29 1.5.1.29] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE= VIBRIO HARVEYI NADPH\:FMN OXIDOREDUCTASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bkj OCA], [https://pdbe.org/1bkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bkj RCSB], [https://www.ebi.ac.uk/pdbsum/1bkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bkj ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bkj OCA], [http://www.ebi.ac.uk/pdbsum/1bkj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bkj RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/FRP_VIBHA FRP_VIBHA] Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/1bkj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bkj ConSurf].
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<div style="clear:both"></div>
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'''NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI'''
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==See Also==
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*[[Flavin reductase|Flavin reductase]]
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__TOC__
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==Overview==
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</StructureSection>
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We report the structure of an NADPH:FMN oxidoreductase (flavin reductase P) that is involved in bioluminescence by providing reduced FMN to luciferase. The 1.8 A crystal structure of flavin reductase P from Vibrio harveyi was solved by multiple isomorphous replacement and reveals that the enzyme is a unique dimer of interlocking subunits, with 9352 A2 of surface area buried in the dimer interface. Each subunit comprises two domains. The first domain consists of a four-stranded antiparallel beta-sheet flanked by helices on either side. The second domain reaches out from one subunit and embraces the other subunit and is responsible for interlocking the two subunits. Our structure explains why flavin reductase P is specific for FMN as cofactor. FMN is recognized and tightly bound by a network of 16 hydrogen bonds, while steric considerations prevent the binding of FAD. A flexible loop containing a Lys and an Arg could account for the NADPH specificity. The structure reveals information about several aspects of the catalytic mechanism. For example, we show that the first step in catalysis, which is hydride transfer from C4 of NADPH to cofactor FMN, involves addition to the re face of the FMN, probably at the N5 position. The limited accessibility of the FMN binding pocket and the extensive FMN-protein hydrogen bond network are consistent with the observed ping-pong bisubstrate--biproduct reaction kinetics. Finally, we propose a model for how flavin reductase P might shuttle electrons between NADPH and luciferase.
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[[Category: Large Structures]]
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==About this Structure==
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1BKJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. This structure supersedes the now removed PDB entry 1CUM. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BKJ OCA].
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==Reference==
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Flavin reductase P: structure of a dimeric enzyme that reduces flavin., Tanner JJ, Lei B, Tu SC, Krause KL, Biochemistry. 1996 Oct 22;35(42):13531-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8885832 8885832]
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[[Category: FMN reductase]]
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[[Category: Single protein]]
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[[Category: Vibrio harveyi]]
[[Category: Vibrio harveyi]]
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[[Category: Krause, K L.]]
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[[Category: Krause KL]]
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[[Category: Lei, B.]]
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[[Category: Lei B]]
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[[Category: TU, S C.]]
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[[Category: TU S-C]]
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[[Category: Tanner, J J.]]
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[[Category: Tanner JJ]]
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[[Category: flavoprotein]]
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[[Category: luminescence]]
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[[Category: nadp]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:02:57 2008''
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Current revision

NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI

PDB ID 1bkj

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