5c05
From Proteopedia
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==Crystal Structure of Gamma-terpinene Synthase from Thymus vulgaris== | ==Crystal Structure of Gamma-terpinene Synthase from Thymus vulgaris== | ||
| - | <StructureSection load='5c05' size='340' side='right' caption='[[5c05]], [[Resolution|resolution]] 1.65Å' scene=''> | + | <StructureSection load='5c05' size='340' side='right'caption='[[5c05]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5c05]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C05 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5c05]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thymus_vulgaris Thymus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C05 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c05 OCA], [https://pdbe.org/5c05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c05 RCSB], [https://www.ebi.ac.uk/pdbsum/5c05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c05 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/GTPS1_THYVU GTPS1_THYVU] Involved in the biosynthesis of phenolic monoterpenes natural products thymol and carvacrol which have a broad range of biological activities acting as antimicrobial compounds, insecticides, antioxidants and pharmaceutical agents (PubMed:26750479). Monoterpene synthase which catalyzes the conversion of geranyl diphosphate (GPP) to gamma-terpinene and the minor products alpha-thujene, alpha-terpinene, myrcene, sabinene, (+)-R-limonene, alpha-pinene and alpha-phellandrene (PubMed:26750479).<ref>PMID:26750479</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The biosynthesis of gamma-terpinene, a precursor of the phenolic isomers thymol and carvacrol found in the essential oil from Thymus sp., is attributed to the activitiy of gamma-terpinene synthase (TPS). Purified gamma-terpinene synthase from T. vulgaris (TvTPS), the Thymus species that is the most widely spread and of the greatest economical importance, is able to catalyze the enzymatic conversion of geranyl diphosphate (GPP) to gamma-terpinene. The crystal structure of recombinantly expressed and purified TvTPS is reported at 1.65 A resolution, confirming the dimeric structure of the enzyme. The putative active site of TvTPS is deduced from its pronounced structural similarity to enzymes from other species of the Lamiaceae family involved in terpenoid biosynthesis: to (+)-bornyl diphosphate synthase and 1,8-cineole synthase from Salvia sp. and to (4S)-limonene synthase from Mentha spicata. | ||
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| + | Expression, crystallization and structure elucidation of gamma-terpinene synthase from Thymus vulgaris.,Rudolph K, Parthier C, Egerer-Sieber C, Geiger D, Muller YA, Kreis W, Muller-Uri F Acta Crystallogr F Struct Biol Commun. 2016 Jan 1;72(Pt 1):16-23. doi:, 10.1107/S2053230X15023043. Epub 2016 Jan 1. PMID:26750479<ref>PMID:26750479</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5c05" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Thymus vulgaris]] |
| - | [[Category: | + | [[Category: Mueller-Uri F]] |
| - | + | [[Category: Muller YA]] | |
| - | + | [[Category: Parthier CP]] | |
| - | + | [[Category: Rudolph K]] | |
| - | [[Category: | + | |
| - | [[Category: | + | |
| - | [[Category: | + | |
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Current revision
Crystal Structure of Gamma-terpinene Synthase from Thymus vulgaris
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